Preparation and application of bifunctional chelates for biological electron transfer studies and preparation of monoclonal antibodies

Iodoacetyl functionality is known to specifically bind methionine 65 residue on cytochrome c. Cytochrome c interacts with cytochrome c oxidase and reductase via the region around this amino acid.;The synthesis of a bifunctional chelate derivative of EDTA with an iodoacetyl side functional group was investigated. The last step in the synthesis of this compound produces two products based on the published methods. Model reactions were conducted to find conditions which produces only the desired product.;Bifunctional chelate derivative of water soluble tetrasulfonatotetraphenylporphyrin was used to generate and isolate antibodies against the metalloporphyrin CoTPPS4. Covalent binding of this metalloporphyrin to bovine serum albumin and keyhole limpet hemocyanin was accomplished by preparing the CoTPPS3C where one of the sulfonate functional groups on the phenlyporphyrin is replaced with a carboxylic acid functionality. The preparation of this metalloporphyrin is described. Several mass cultures were isolated and screened by ELISA. Mass cultures 2B5, 2B9 and 2C3 were specific for the antigen, CoTPPS3C. 2B9 mass culture was cloned and a monoclonal antibody was isolated which proved to be highly specific against CoTPPS3C.;In the following section the electrochemistry of TPPS4 and CoTPPS4 was investigated in aqueous solution at pH range of 1-14 by using various electrodes. Following, the electrochemistry of this metalloporphyrin was investigated by using several modifiers and promoters.;The electrochemistry of CoTPPS4 and TPPS4 in DMF/TEAP was investigated. The Diffusion coefficient and heterogeneous rate constant of CoTPPS4 and TPPS4 were calculated by using a working planar gold electrode. Finally the electrochemistry of CoTPPS4 in DMF/H;The role of tetramethylpyridiniumporphyrin (TMPy) and its Co...