| Although all women experience postmenopausal bone loss, only 25-30% will develop postmenopausal osteoporosis, therefore another mechanism must be working with estrogen deficiency to cause this disease. Our proposed mechanism outlines the accumulation of advanced glycation endproducts (AGEs) as the driving force behind bone loss through intracellular signaling after ligation to the receptor for advanced glycation endproducts (RAGE). The study employed a high-fructose diet to initiate production of AGEs and investigated the effects on bone loss and RAGE expression after ovariectomy. This study investigated changes in bone quality and oxidative damage due to diet and surgeries as well as levels of soluble and bone collagen bound AGEs. It also worked to evaluate RAGE expression in serum and osteoclasts. Results show there was no production of AGEs, and bone quality and oxidative damage were affected by ovariectomy only. Soluble RAGE expression was increased due to ovariectomy alone, however cell-bound RAGE was unchanged. |
