Microbiota Colonization In Newborn Rats And Effects Of Pu'er Born Tea On Internal Microbiota

Many studies have shown that many microorganisms are present in the human intestinal tract,and these microorganisms are involved in various metabolic regulation in the body.In addition,there are different microbial communities in the surface(skin,hair,etc.)of the normal human body and in the cavity communicating with the outside(nasal cavity,etc.);and microbes are also found in other organs of the body,and these microorganisms have correlation with diseases.However,the source of microbes in human body is still unclear.In this paper,colonization of microbiota in newborn individuals,the changes in microbiota along with the growth in differnt phases,and the changes in microbiota by the differences of diet were studied.It is generally believed that the fetus is sterile in the uterus,and the colonization of microorganisms in the body and intestinal tract begins only after birth.However,it is known that there are abundant microbial flora in organs of the mother,including the placenta and amniotic fluid,so it can be reasonably speculated that the microbial colonization of fetus begins in the prenatal period.In this study,the 16 S r RNA high-throughput sequencing technology was used to identify the microbiota in the blood and gut of prenatal maternal,neonatal,and postpartum rats.It was found that there were 20 kinds of bacteria in maternal placenta,amniotic fluid and umbilical cord,such as Firmicutes,Bacteroides and Proteobacteria.There were 19 species of bacteria at the family level,including Lachnospiraceae,Prevotellaceae,Burkholderiaceae,etc.Bacteroides,Lachnospira,Prevotella,and 18 species of bacteria at the genus level.At the phylum level,the uterine microflora completely contained the microflora in the newborn,which indicated that the microflora in the newborn mice was transferred from the microflora in the maternal uterus.The results also suggested that the colonization of individual blood and intestinal microbes begins before birth,other than post-birth.This demonstrated that neonatal individuals are not sterile,although they were thought to be germ-free traditionally.Along with growing up,the blood and intestinal microbiota of the rats change,especially during the weaning period.The microbiota abundance and structure are then gradually approached to the that of the mother.In recent years,researches on gut microbiome has been very popular,and achieved lots of findings,and gut microbiota were thought to related with obesity,diabetes,and other health conditions or diseases.Diet is recognized as one of the most important factors to easily alter gut microbiota.The difference in diet,that is,amino acids,vitamins,etc.,directly affects the structure of the microflora in the gut,disturbs the original balance of the gut,and can successively present various metabolic diseases.In this paper,weaned rats were fed with tea,and the blood and feces of rats were taken under aseptic conditions.16 S r RNA High-throughput sequencing technology was used to analyze the changes of microbiota in blood and gut of rats.The results showed that the microbial abundance in blood and gut of the mice fed with tea significantly changed,and the change was related to their body weight.