Effect Of Microbial Compound Agents On Rhizosphere Soil Microbiome In Rice

In order to achieve the sustainable development of agricultural production and reduce the use of chemical fertilizers and pesticides,green,environmentally friendly and eco-friendly microbial inoculants have been widely used worldwide.microbial compound agents are stronger and more stable than single bacteria,which is the development trend of microbial agents.At present,there is a lack of research on the construction method of compound fungicides and the research on the growth promotion mechanism of compound fungicides in rhizosphere soil.In this paper,pot experiment was used to screen the high-efficiency microbial compound agent FN and study its ecological mechanism to promote rice growth in soil,especially the effect on the rhizosphere soil microbiome.The main results and conclusions are as follows:（1）The effect of the high-efficiency microbial compound agent FN on rice growth was significantly better than that of the blank control CK and the commercial microbial compound agent M,and the growth promotion effect was stable in the field.A mathematical model of the microbial compound agent was constructed,and the growth-promoting effect of the microbial compound agent on rice could be predicted by the growth-promoting characteristics of the microbial species,and the prediction accuracy rate could reach more than 97%.（2）The ecological mechanism of compound agent FN in promoting rice growth in soil was further studied.The results showed that N,F and FN in the treatment group could significantly promote the uptake of nitrogen,phosphorus and potassium in rice,increase the biomass of rice and promote the growth of rice,among which the growth promoting effect of compound bacterial agent FN was significantly better than that of single bacterial agent N and F（P<0.05）.（3）Treatment groups N,F and FN could significantly change the physical and chemical properties of rice rhizosphere soil,increase the number of bacteria and fungi in rhizosphere soil,and the FN could significantly significantly increase the abundance of Bacillus in rhizosphere soil.（4）Compound agent FN can significantly change the structure and function of the soil bacterial community,improve the bacterial richness and diversity,and enrich the subgroup₆,thermophilic,planctomycetacia,deltaproteobacteria and other species（P<0.05）.Compound agent FN had no significant difference in the structure of soil fungal community,but significantly increased the fungus richness,and enriched the species such as ascobolaceae,pleosporaceae and pyronemataceae（P<0.05）.Correlation analysis showed that treatment group N,F,and enrichment of FN related species and species diversity were positively correlated with rice most features,and treatment group N,F and FN can change soil physical and chemical properties and promote rice growth and development,the enrichment of speculation related species could be in soil nutrient cycling and nutrient uptake of rice plays an important role,and by promoting rice on the absorption of nitrogen,phosphorus and potassium nutrient elements to promote rice growth.In summary,the growth promotion effect of the compound bacterial agent in this paper is better than that of the single bacterial agent,which may be related to the fact that the compound bacterial agent FN can promote the absorption of nutrients in rice and the beneficial species with the highest bacterial richness,diversity and enrichment.The application of compound agent FN can change the physical and chemical properties of soil to some extent,significantly change the structure and function of microbial community,enrich beneficial species,significantly promote the absorption of nitrogen,phosphorus and potassium nutrients by rice,promote the growth and increase production of rice,and promote the growth effect to be stable.Therefore,we speculated that the compound bacterial agent FN mainly regulates the structure and function of rhizosphere microbial community to promote nutrient absorption and growth of rice.