Transcriptome And Proteome Analyses Of Arabidopsis Thaliana Undergoing Low PH Stress

The low pH environment and Al stress in acid soil can cause damage to plants,both of which can inhibit root elongation.The mechanism of plant tolerance to low pH and Al stress has been a hot topic in recent years,but compared with the research on metal ion Al,the effect of low pH on plants has not been studied in depth.In this paper,from the perspective of combined analysis of TMT-labeled quantitative proteome and transcriptome,the protein expression changes and gene expression changes of Arabidopsis under low pH conditions were studied,and low pH-responsive genes and proteins were identified and screened.At the same time,through gene cloning and transformation method,we transformed the important low pH-related genes found in omics into Arabidopsis thaliana,observed the low pH-related phenotypes of Arabidopsis thaliana,and performed functional verification of the related genes.Important research results are as follows:1.In the proteome,there are 64 unique differential proteins in the 6 h treatment group,94 unique differential proteins in the 24 h treatment group,and only 12differential proteins identified in the 6 h and 24 h treatment groups.2.In the transcriptome,a total of 712 differentially expressed transcripts were obtained,including 224 up-regulated genes and 488 down-regulated genes.There were more up-regulated genes than down-regulated genes.3.Through functional enrichment analysis of differentially expressed proteins in the proteome,we found different proteins in the 6 h treatment group of the proteome were mainly enriched in chromatin assembly and disassembly,nucleosome assembly and DNA recombination process pathways and the differential proteins in the 24 h treatment group were enriched in photosynthesis,energy synthesis and photoelectron transmission pathways.4.The differentially expressed genes of the transcriptome are mainly enriched in response to organonitrogen compound,hydrogen peroxide response,chitin response,protein folding,protein complex oligomerization,cell response to reduced oxygen levels,carbohydrates derivative catabolic process and amino sugar catabolic process.5.The correlation between replicates in the experimental treatment group approached 1,but the correlation between the transcriptome and the proteome was only about 0.05,indicating that the correlation between changes in the proteome and the transcriptome under 24 h low pH treatment was low.6.In the study of the root length phenotype of transgenic Arabidopsis,we found that compared with the wild-type Col4,the root lengths of the seedlings overexpressing the carbohydrate metabolism-related genes GAPB(AT1G42970)and SBPASE(AT3G55800);hormone response related genes SAUR58(AT1G43040)and GRP3(AT2G05520);photosynthesis related gene PSAG(AT1G55670)and photorespiration related gene AGT(AT2G13360),using 35S promoter(cauliflower mosaic virus promoter),were significantly shorter.7.Through the measurement of luciferase activity,the luciferase activity of35S::LUC-HFP108(AT3G63190)and 35S::LUC-GRP3(AT2G05520)transgenic Arabidopsis thaliana was significantly lower than that of the control 35S::LUC under low pH treatment.8.The low pH treatment conditions significantly increased the content of chlorophyll b(C_b)in Arabidopsis thaliana,resulting in a significant difference in C_a/C_b,indicating that low pH treatment can affect the ratio of chlorophyll components in Arabidopsis.In su mmary,through the combined analysis of transcriptomics and proteomics in Arabidopsis under low pH treatment conditions,we screened out important low pH response proteins and genes,and gained a preliminary understanding of the analytical level regulatory network of low pH stress and conduct transgenic seedlings verification and functional analysis of important stress genes.