Prussian Blue Analogues Composite Material As Peroxidase Mimetics And The Ir Application In Small Biological Molecules

Natural enzymes play an important role in various fields because of their high specificity.However,the intrinsic drawbacks and high cost of natural enzymes limit them development.In order to solve these problems,researchers have been devoted to the development of mimic enzymes.The mimic enzymes have been widely used in many fields due to their low cost,higher stability,simple preparation,tunable catalytic activity and reusability.Compared with other artificial enzymes,nanomaterials as mimic enzymes have outstanding properties such as their large specific surface area,controllable size and morphology,such as metal nanoparticles,transition metal oxides,metal sulfides,metal organic frameworks,carbon materials,composite nano materials.A stable,fast,and highly sensitive analysis method can be constructed through the combination of mimic enzymes and different analysis methods.Among these analysis methods,the colorimetric analysis method and the chemiluminescence analysis method have been widely used because of their low cost,simple operation,and rapidity.Based on these,this study concentrated on prepared a kind of Prussian blue analogue nanoparticles used as peroxidase mimetic enzyme,enhancing its catalytic activity by compounded graphene oxide,which were applied in colorimetry method and chemiluminescence method to the detection of disease markers.Based on this,this study synthesized a Prussian blue-like nanoparticle with peroxidase activity and further enhanced its catalytic ability by compounding graphene oxide,which was then combined with colorimetric and chemiluminescent assays for the detection of hydrogen peroxide and disease markers.This article mainly includes the following parts:1.Prussian blue analogue nanoparticles(CoFe(?)PBA-2)were prepared.Compared with horseradish peroxidase(HRP),CoFe(?)PBA-2 has a stronger affinity toward hydrogen peroxide and 3,3',5,5'-tetramethylbenzidine(TMB).We constructed a highly colorimetry method to the detection of hydrogen peroxide.The linear range of this method for the determination of hydrogen peroxide is 0-120?mol/L,and the detection limit is 4.30?mol/L.After that,we further combined with cholesterol oxidase to achieve cholesterol detection.The linear range of this method for determining cholesterol is 0-80?mol/L,and the detection limit is 1.30?mol/L.This method has been successfully applied to the detection of hydrogen peroxide in hydrogen peroxide disinfected water and cholesterol in human serum.2.In this study,we prepared a Prussian blue/graphene oxide composite nanomaterial(CoFe(?)PBA/GO)with peroxidase activity.Compared with GO and CoFe(?)PBA-2,CoFe(?)PBA/GO has stronger affinity for hydrogen peroxide.Using it as a catalyst,we have established a colorimetric analysis method for detecting hydrogen peroxide.The linear range of this method for the determination of hydrogen peroxide is 0-24?mol/L,and the detection limit is 0.96?mol/L.On this basis,we further combined with cholesterol oxidase to achieve a highly sensitive detection of cholesterol.The linear range of this method for determining cholesterol is 0-32?mol/L,and the detection limit is 1.7?mol/L.Moreover,the method can be applied to the detection of hydrogen peroxide in hydrogen peroxide disinfected water and cholesterol in human serum.3.A chemiluminescence method for the detection of hydrogen peroxide and ascorbic acid(AA)was constructed by combining CoFe(III)PBA/GO with peroxidase activity.CoFe(?)PBA/GO can catalyze H₂O₂ to produce O₂·^-,·OH,~1O₂ radicals to oxidize Luminol to produce strong chemiluminescence signals.By detecting the intensity of chemiluminescence,a highly sensitive detection of hydrogen peroxide was achieved.The linear range of this method for detecting hydrogen peroxide is 0-0.8?mol/L,and the detection limit is 0.011?mol/L.On this basis,we used ascorbic acid(AA)as an active oxygen scavenger,which can inhibit the chemiluminescence reaction and realize the detection of AA.The linear range of AA determined by this method is 0.02-0.8?mol/L,and the detection limit is 0.02?mol/L.This method has been successfully applied to the detection of ascorbic acid in hydrogen peroxide and vitamin C tablets in hydrogen peroxide disinfection water.