Analysis On The Regulation Of Endogenous Hormone Pathway Genes In Different Development Modes Between Male And Female Of Ericerus Pela(Hemiptera:Coccidae)

Ericerus pela(the White Wax Scale Insect,WWS)is an important resource insect.Its second instar male secretes a large amount of biomass wax which is a natural polymer compound and its main component is 26-acid 26-lipids.Biomass max has been widely used for printing,machinery,medicine,food,new materials,and other fields,and has important economic value By analyzing the transcriptome data of WWS,the different gene expression rules of the WWS were expounded,to reveal the difference between male and female development of the WWS,and to explain the regulation of KEGG pathway genes of endogenous hormone on the development of the WWS.Here are the main research results:1.The dynamic expression rule of the transcriptome of Ericerus pelaThe second instar male nymph(SM)has 17,296 differentially expressed genes compared with the first instar male nymphs(FM).SM has 16,308 differentially expressed genes compared with SF,and 8620 and 5968 specific different genes in SM vs FM and SM vs SF,respectively.The organic substances and other biological metabolic processes in SM are significantly enriched.The biological metabolic processes such as chitin and glucosamine complex in SF are enriched.Therefore,there is a significant difference in physiology and biochemistry between the male and female second instar nymphs of WWS,the different development of male and female begin in the second nymph,and follow a specific developmental direction.2.Analysis of KEGG metabolic pathway of endogenous hormone in WWSBy analyzing the KEGG metabolic pathway of endogenous hormone,we found that the key genes of juvenile hormone(JH)and molting hormone(20E)metabolic pathways significantly differentially expressed in SM and SF.In SF,the key gene of molting hormone synthesis(CYP307A1 CYP314A1)is low expressed,while the key gene of molting hormone hydrolysate(CYP18A1)is high expressed;The key gene for the synthesis of juvenile hormone(CYP15A1)is highly expressed,and the key gene for the hydrolysis of juvenile hormone(JHE)is poorly expressed,so the study forecasted molting hormone titer is low and juvenile hormone is high in SF,the interaction between 20 E and JH in SF is enhanced,and JH played a leading role.SF begins to develop into incomplete metamorphosis,from SF to female adult.In SM,the key gene of molting hormone synthesis(CYP307A1 CYP314A1)is highly expressed,while the key gene of molting hormone hydrolysate(CYP18A1)is poorly expressed;The key gene for the synthesis of juvenile hormone(CYP15A1)is poorly expressed,and the key gene for the hydrolysis of juvenile hormone(JHE)is highly expressed,so the study forecasted molting hormone titer is high and juvenile hormone is low in SM,the interaction between 20 E and JH in SF is weakened,and 20 E played a leading role.SM begins to develop into complete metamorphosis,from SF to prepupa.3.Gene function analysis of CYP18A1CYP18A1 plays a vital role in insect metamorphosis and development.CYP18A1 is the 20 E hydroxylase,which is involved in the 20 E deactivation process and maintains the 20 E balance in the insect body.In this paper,the transcription sequence of CYP18A1 was obtained and its bioinformation was predicted,and it was found that the expression abundance of CYP18A1 protein is low and it is easy to be inactivated and unstable.CYP18A1 is regularly and differentially expressed with the metamorphosis process in the body of WWS,and coordinated to regulate the metamorphosis development of WWS.After the CYP18A1 of the second male nymph was knocked out,the development of the WWS was deformed,some of the nymphs died.we confirmed that CYP18A1 gene is involved in the process of ecdysis and plays an important regulatory role in the development of complete metamorphosis in males.This study through the analysis of WWS transcriptome expression of dynamic,reveals WWS transcriptome expression of dynamic rule,explained the starting point which the sexual dimorphism development phenomenon of WWS,at the same time analyzed the JH and 20 E metabolic pathways differences in gene expression pattern.A model for the regulation of insect hormones on the development of WWS was proposed.This provides a molecular basis for the subsequent analysis of the phenomenon of sexual dimorphic in insects.Meanwhile,we screened the key genes of 20 E inactivation and signal transduction pathway,and revealed their influence on the growth and complete metamorphosis development of the second male nymph of WWS through RNAi,which provided a certain basis for the study of the development of male and female dimorphism in WWS and other insects,and provided a scientific basis for the breeding of high-yielding varieties of WWS.