| The increasingly serious problem of water shortage has promoted the rapid development of the desalination industry.While alleviating the shortage of fresh water resources,it also produces a large amount of high-salinity wastewater,namely concentrated brine.At present,the disposal of concentrated salt water is mainly based on direct discharge to the sea.A large amount of concentrated salt water entering the ocean will inevitably cause a series of ecological problems,and it is also a huge waste of resources.In this paper,a series of exploration experiments are carried out on the concentrated brine discharged from the reverse osmosis seawater desalination process,a seawater desalination technology with a high market share.Combining the high-salt characteristics of concentrated brine with the living conditions of Artemia and Dunaliella salina,optimizing the conditions for breeding Artemia in concentrated brine,and exploring the passage of Artemia,providing a favorable basis for large-scale culture of Artemia.It forms a good combination mode with the use of concentrated brine.The body length and survival rate of different generations of Artemia were continuously observed.It was found that the average body length of the fifth-generation Artemia was the longest,with an average body length of 8.993 mm at 60 d,but its survival rate was low.The survival rate at 30 d was about 42 %,the fourth-generation Artemia is closely followed by the average body length,which can reach 8.529 mm in 60 d,and the30 d survival rate is about 53 %.The body lengths of the first three generations of Artemia are similar,with an average of 8.413 mm,and the survival rate at 30 d is about 70 %.Compared with the fourth and fifth generations of Artemia,there are big differences.Observing the reproduction status of different generations of Artemia,it was found that the number of nauplii produced by the first generation of Artemia was about 8,409,the second generation was about 7,947,and the third generation was about 7,859.Compared with the previous three generations,the number of nauplii produced by the fourth generation Artemia showed a linear decline,about 2,051.Control the temperature at 5 ?,10 ? and room temperature to explore the growth rate and reproduction cycle of Artemia.Under the condition of 5 ?,under the severe influence of temperature,although the bait is abundant,whether it is light or protected from light,at 10 ?,the survival rate of Artemia in the illuminated group is higher than that of the dark group,and under 65 d of light Female Artemia have metabolic offspring.In the light-shielding group,the low temperature group is compared with the room temperature group.The cycle of female Artemia from conceiving to triggering offspring under low temperature accumulates,about 30 d,and about 5 d.Combining the experimental results,the third,fourth,and fifth generation mature individuals of Artemia were transformed into the transcriptome and analyzed through the transcriptome.In this SSR analysis of Artemia transcription and transformation results,a total of 13,758 Unigenes appeared.A total of 6,256 SSR sites were detected in the sequence from single nucleotide to hexanucleotide repeat types,and the frequency of SSR sites was about 45.47 %.The estrogen of different generations of Artemia,and the correlation value between males is greater than 0.8,which indirectly indicates that the more repeated samples between samples,the more reliable the differentially expressed genes that can be found.In the annotation of differentially expressed genes KEGG,the glycolysis/ gluconeogenesis pathway annotation is at most 7,accounting for 8.05 % of the total number of annotated genes.The significant increase in glycolysis/gluconeogenesis pathways can better convert substances into sugars and provide nutrients for the growth of Artemia. |
PDF Full Text Request