Research On The Regulation Of Biological Macromolecular Function Based On Graphene Oxide/Conjugated Polymer

Conjugated polymers are widely used in regulation of biomolecules or small molecule due to their good biocompatibility and fluorescence amplification.In this paper,we have designed a variety of materials with photothermal conversion ability by taking the advantages of conjugated polymer and graphene oxide(GO),and studied its application in DNA-protein interaction regulation and carbon dioxide(CO₂)adsorption.The main research contents include:1.A new method for photothermal detection and regulation of DNA-protein interactions was designed by using a composite system of GO and conjugated polymers.Under the irradition of near-infrared light(NIR),GO can cause increase in the temperature of the solution and denaturation of ds DNA while quenching the fluorescence of fluorescein-labeled DNA.The fluorescence energy resonance transfer(FRET)effect between the water-soluble conjugated polymer(PFP)and the DNA greatly increase the sensitivity of the assay.After the addition of single-stranded binding protein(SSBP),ss DNA binds to SSBP that lead to recovery of FRET.This method effectively regulated the interaction of DNA-SSBP and detected fluorescence changes by simply using NIR light.2.A new pyrrolopyrroledione(DPP)conjugated polymer material(PDPP-Gu)that has a photothermal effect to control the release of CO₂ was designed and synthesized.PDPP-Gu is composed with strong electron donor-acceptor units in the molecular structure,which results in strong light absorption ability near 800 nm.Therefore,the polymer exhibit excellent photothermal conversion ability and high photothermal conversion efficiency.Moreover,PDPP-Gu has a higher surface energy with a structure of guanidyl,which makes this polymer a good selective adsorbent for CO₂.The adsorption capacity reaches 0.8mmol/g at 273K 1 bar.The polymer has the dual characteristics of CO₂ adsorption and photothermal conversion.It can dynamically control the adsorption and release of CO₂ by NIR irradition,which shows the excellent application prospect of DPP conjugated polymer in CO₂ adsorption.