Mechanism Of Degradation Of Bisphenol F By White-rot Fungi And Bioremediation Application

Bisphenol A(BPA)is one of the largest compounds used for industry in the world,some countries and international organizations have regulated the production and use of BPA because of its endocrine disruption effect.Bisphenol F(BPF)is the most commonly "BPA alternative" in industrial production.However,BPF has also been proven that it has comparable endocrine interference activity to BPA and has caused environment pollution.Microbial remediation is the most promising way to treat toxic pollutants in the environment.White rot fungi have attracted wide attention because of their broad-spectrum and high-efficiency in the degradation of organic pollutants.The current research on the degradation of BPF by white-rot fungi is mainly focused on the degradation mechanism of BPF by fermentation or laccase treatment.There is little knowledge about the BPF-degrading.In this paper,the white-rot fungus Phanerochaete sordida YK-624 was used to study the degradation ability and degradation mechanism under different degradation conditions.RNA-seq was used for detecting the BPFdegradation genes in P.sordida YK-624.In addition,BPF-contaminated wastewater was treated by P.sordida YK-624 for investigating the feasibility of the application of white-rot fungi in bioremediation.The main results obtained in this study are as follows:(1)P.sordida YK-624 has well ability to degrade BPF under ligninolytic conditions and non-ligninolytic conditions.P.sordida YK-624 could degradation 99.24% of BPF after 4 d incubation under ligninolytic condition with p H of 4.5,temperature of 30 ? and BPF concentration of 0.1 m M.Under non-ligninolytic condition,BPF could also be completely degraded after 7 d at natural p H,temperature of 30 ? and BPF concentration of 0.1 m M.(2)According to the effect of cytochrome P450 experiments and transcriptome analysis results,Li P,Mn P and cytochrome P450 might be involved in the degradation of BPF by P.sordida YK-624 under ligninolytic conditions;and cytochrome P450 played an important role in BPF degradation under non-ligninolytic condition.(3)4,4'-dihydroxybenzophenone(DHBP)was detected as BPF metabolite by P.sordida YK-624 under non-ligninolytic condition by MS.With the quantitatively evaluating of BPF and DHBP,it has observed that the concentration of DHBP increased at first 3 d after BPF consumption decreased.(4)Treatment of BPF-contaminated wastewater by P.sordida YK-624 under static and shaking conditions was implemented.Under static conditions,98.3% BPF could be removed after 5 d incubation.Under shaking conditions,88.3% of BPF could be removed after 7 d incubation.The biomass of P.sordida YK-624 showed a downward trend during the treatment.According to the activities of lignin degrading enzymes,manganese peroxidase was involved with the degradation of BPF in wastewater by P.sordida YK-624.