Effect Of Iron Restriction Induction On The Transcriptome Of Synechococcus

Synechococcus is one of the most abundant autotrophic prokaryotes living in oceans and lakes.It has made great contributions to the carbon cycle and oxygen supply in the ecological environment,and is an important member of the ecosystem that cannot be ignored.Although it is an autotrophic organism,it is very dependent on some inorganic elements and solar energy,such as iron,nitrogen,potassium,and phosphorus.Transcriptome is a good way to study gene expression.It can understand the source of the expression of living organisms.It is the basis and starting point for the study of gene function and structure.The transcriptome is an important tool and method for researching and discovering functional genes.It can study gene function and gene structure at the overall level,revealing specific biological processes and molecular mechanisms in the process of disease occurrence.In order to understand how iron plays a role in the physiological functions of Synechococcus,transcriptome analysis was performed by comparing the effects of different concentrations of iron on the transcriptome of Synechococcus sp.PCC 7002.Three different iron concentrations were used for processing,and each group of data was repeated three times for a total of nine groups of experimental data;the control group had a concentration of 10.9 n M,and the iron-restricted group was 0.41 n M and0.003 n M,respectively.It is found that under the iron-restricted environment,Synechococcus can cope with the iron-deficiency environment by increasing the extracellular iron uptake,up-regulating the expression of photosynthesis-related proteins and adjusting the metabolic pathways,and the degree of iron-restriction is positively correlated with the number of differential genes.In addition,strong iron deficiency affects the ability of Synechococcus sp.PCC 7002 to resist foreign virus infection by down-regulating the expression of certain nucleases in the CRISPR system,and the expression level of the ABC transporter that transports the phosphate group has also decreased.This greatly affected its survival.This paper mainly discusses how Synechococcus sp.PCC 7002 responds to such stress at the transcriptome level under different levels of iron restriction,so as to adapt to the pressure of iron deficiency at the whole biological level by studying the transcriptome of pylorella under iron restriction,we can understand how different iron restriction affects the viability of pylorella,and increase the understanding of the adaptation mechanism of pylorella to this stress environment,which provides ideas for the future transcriptome research of Marine microorganisms and other aquatic microorganisms under iron restriction.