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Inhibition Of Lactobacillus Plantarum UD01 On Formation Of Fusobacterium Nucleatum Biofilm And Its Consumption Of Odor-causing Substrates

Posted on:2021-12-05Degree:MasterType:Thesis
Country:ChinaCandidate:Y T ZhongFull Text:PDF
GTID:2480306503991619Subject:Bio-engineering
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Fusobacterium nucleatum(F.nucleatum)is one of the main pathogenic bacteria which can cause pathological halitosis.Forming biofilm is the key in the mechanism of F.nucleatum causing bad breath besides producing volatile sulfur compounds(VSCs).Probiotics and other biological therapies have gradually attracted researchers' attention because bad breath is more likely to return after traditional methods which also produce many side effects.In this study,we used cell-free supernatant(CFS)that from different probiotics with a p H of 6.6 to treat with F.nucleatum,in order to screen the probiotic which can inhibit the formation of F.nucleatum biofilm with an oral neutral p H.Through crystal violet semi-quantitative method,Lactobacillus plantarum UD01 was selected for its outstanding ability in reducing the F.nucleatum biofilms.The minimum biofilm inhibition concentration(MBIC)of the UD01 CFS(p H 6.6)assayed by the microdilution method was 250 ?L/m L.Scanning electron microscope(SEM)and confocal laser scanning microscope(CLSM)results showed that F.nucleatum biofilm decreased and became thinner,besides,its structure was looser when treated with UD01 CFS(p H 6.6).Growth curve,real-time quantitative PCR(q PCR)of genes for adhesion-associated outer membrane proteins,and extracellular polymeric substances(EPSs)results indicated that UD01 CFS(p H 6.6)could not inhibit the growth of F.nucleatum,and its possible action mechanism against F.nucleatum biofilm was to downregulate the expression of adhesion-associated outer membrane protein genes,reduce the secretion of extracellular protein and extracellular polysaccharide,thereby reduce the adhesion ability of F.nucleatum,and ultimately inhibit F.nucleatum biofilm.The CFS of L.plantarum UD01 which selected in this study can inhibit the formation of F.nucleatum biofilm with an oral neutral p H.L.plantarum UD01 could be used as a potential probiotic strain for treating halitosis.
Keywords/Search Tags:halitosis, Fusobacterium nucleatum, probiotics, biofilm, adhesion ability
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