Synthesis Of Two Nano-Enzymes Derived From Metal Organic Framework And Its Simulation Enzyme Activity Research And Application

The enzyme has the advantages of high catalytic efficiency and good specificity,and has excellent catalytic activity and regulation ability in aqueous media.A few natural enzymes are nucleic acid molecules,and most are proteins.Its easy inactivation,easy digestion by proteases,high cost and strict storage conditions limit its application.So far,a variety of mimics that can mimic the structure and function of natural enzymes have appeared,including metal complexes,polymers,and other biomolecules.Among them,nanozymes have great potential,they have the advantages of good stability,easy mass production,low synthesis and storage costs,and long-term storage.Therefore,nanozymes have very promising development prospects.The research work of this paper is mainly to synthesize two new nanomaterials for simulating peroxidase and oxidase,and for colorimetric analysis to detect biomolecules and ions.The main results are as follows:1.Metal-organic framework(MOFs)nanosheets(NS;ZIF-67 type)were prepared by ultrasonic hydrothermal method using 2-methylimidazole,manganese(II)and cobalt(II)as precursors.The doping rate of Mn(II)is as high as 11.3%.The nanosheet inherits the advantages of the metal organic framework(MOFs)such as high porosity,large specific surface area and a large number of active sites.It can directly catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine(TMB)by molecular oxygen to form a blue ox TMB.Based on this feature,a simple and rapid colorimetric analysis method for the determination of GSH and glutathione reductase(GR)activity was established,and the GSH content in human serum was detected by the spike recovery method.The experimental results show that GSH and GR are linearly correlated in the concentration range of 0.1?25?mol/L and 0.1?3 m U/m L,respectively.The detection limits are 0.07?mol/L and 0.18 m U/m L,respectively.And the recovery rate of GSH in human serum is 96.42?122.60%.The above results indicate that the system is practical in biomolecule detection applications.2.We have designed a new type of hollow Mn Fe O oxide with oxidase-like activity and peroxidase-like activity,which can easily adjust its double enzyme catalytic activity under different p H conditions.And used for colorimetric detection of cysteine(Cys),Hg²⁺and H₂O₂.Experimental results show:The colorimetric detection of Cys and Hg²⁺under the condition of p H=3.5,the linear detection range is 1?25?mol/L and 0.1?15?mol/L.The detection limits were 0.04?mol/L and 0.02?mol/L,respectively.The colorimetric detection of H₂O₂ under the condition of p H=4.5,the linear detection range is 1?60?mol/L and60?300?mol/L,and the detection limit is 0.05?mol/L.The Cys content in fetal bovine serum and the Hg²⁺content in water samples were detected by colorimetric detection through spike recovery method.The recovery rates were 104.8?111.8%and 108.2?112.7%,respectively.