Synthesis Of Fluorescent Probes Based On TICT And Application Of Human Serum Protein Detection And Targeted Lipid Droplet Imaging

Human serum albumin(HSA)has important physiological and pharmacological functions,and accurate determination of its concentration is of great significance for the pre-diagnosis of specific diseases.The triphenylamine structure and the N-N dimethylamino group have good ability to donate electrons.Connecting it and electron withdrawing group through double bondthe,molecular structure has TICT characteristics.When the probe entered the low-polarity hydrophobic cavity of HSA,TICT was inhibited and the fluorescence intensity increased to achieve the purpose of detecting HSA.In addition,the triphenylamine structure is a good targeting group for lipid droplets,so probes with triphenylamine structure could achieve specific imaging of lipid droplets.Chapter 1:This chapter mainly introduced the research progress of HSA fluorescent probe,focusing on the recent research of HSA fluorescent probe based on TICT mechanism.In addition,triphenylamine-based fluorescent probes targeting lipid droplets and their specific imaging were also discussed.Based on this,the background,research content and innovation of this thesis were proposed.Chapter 2:A novel fluorescent probe TPA-CPO based on TICT was designed and synthesized.TPA-CPO could sense HSA with excellent properties including long emission wavelength,large stokes shift,and wide linear range.When TPA-CPO entered the IB subdomain of HSA,its TICT process was inhibited,which lighted up the fluorescence of TPA-CPO and achieved the purpose of selective detection of HSA.In addition,TPA-CPO has been successfully applied to the imaging of serum proteins in He La cells,and TPA-CPO had a good targeting ability to lipid droplets.Chapter 3:A water-soluble fluorescent probe TPA-RDN based on the triphenylamine group was designed and synthesized.After TPA-RDN interacted with HSA,it showed a"turn-on"red fluorescence response due to the change of the microenvironment.In PBS(p H=7.4,20 m M)buffer solution,TPA-RDN had high selectivity and sensitivity for HSA detection.It could quantitatively detect the concentration of HSA in urine and realized to HSA imaging in Hela cells.On the other hand,lipid droplet-specific bioimaging guided by polarity showed that TPA-RDN can target lipid droplets and identify cancer cells.Chapter 4:The probe PA-CPO with longer conjugate structure was synthesized utilizing the CPO group(Chapter 2)and4-(Dimethylamino)cinnamaldehyde.After entering the IB subdomain of HSA,the fluorescence was lighted up due to the TICT mechanism.The detection of HSA was achieved with high sensitivity and could be used for the detection of HSA concentration in actual sample urine.Compared with TPA-CPO(Chapter 2),the emission wavelength was red-shifted;the response was faster that can be completed within 3 minutes;the response was more sensitive that the fluorescence intensity increased by 44 times after adding 1 mg/m L HSA and the detection limit was 1.05?g/m L.In addition,there were linearity in two concentration ranges of 0.01-0.09mg/m L(R~2=0.9941)and 0.09-0.79 mg/m L(R~2=0.9962).