Proteomic Analysis Of Root System Of Different Tolerance Peanut Varieties Under Waterlogging Stress

Peanut is the main oil and cash crop in south China.Frequent waterlogging disasters in spring and summer seriously affected peanut production.The root system of peanut is the most directly and seriously affected by waterlogging.It is of great significance for sustainable development of peanut production to study the waterlogging tolerance mechanism of root system.In this study,a sensitive variety Zhonghua no.4 and waterlogging resistant varieties Xianghua 2008,Quanhua no.8,Yuhua 15 were studied.Root waterlogging(3,6,9,12,15 days)and normal irrigation were carried out during the peanut “four leaf-one bud” stage.Data independent acquisition technology was used to analyze proteome differences and further explore the waterlogging tolerance mechanism of peanut roots.The main research results are as follows:1.Under waterlogging stress,the ADH activity of the taproot of zhonghua no.4increased from day 9 to day 15.The ADH activity of taproot on the 12 th day of waterlogging was significantly higher than that of the control group.The lateral root is different from the taproot.The ADH activity of taproot and lateral root of Xianghua 2008,Quanhua no.8,Yuhua15 reached the peak on the 12 th day of waterlogging and decreased in the later period.Therefore,the root system on the 12 th day of waterlogging treatment was selected as a proteomics sample.2.By using DIA technology,3097 differentially expressed proteins were identified through the comparison of 4 varieties' flooding and normal irrigation.Among them,1993 differentially expressed proteins were identified in the taproot,457 were up-regulated and1536 were down-regulated.1104 differentially expressed proteins were identified in lateral roots,389 were up-regulated and 715 were down-regulated.There were significant differences in protein expression between different cultivars,with yuhua 15 being the most abundant and zhonghua 4 being the least,indicating more mechanisms and strategies for tolerant cultivars to cope with waterlogging.3.GO functional enrichment analysis showed that the differentially expressed proteins in the taproots and lateral roots of the four peanut varieties were mainly concentrated in biological processes such as metabolism and cell process,mainly located in cell membrane,cell membrane components,cells,cell components and other cell components,with catalytic and transport activities and other molecular functions.4.KEGG,KOG enrichment analysis and subcellular structure localization showed that the differentially expressed proteins of primary and lateral roots were mainly enriched in the metabolic pathways of carbohydrate,amino acid,lipid metabolism,secondary metabolites synthesis,transport and decomposition,as well as energy production and conversion,as well as in cell signal transduction and other processes.The subcellular structure classification of differentially expressed proteins was mainly located in chloroplast cytoplasm and plasma membrane.This indicates that the waterlogging resistance of peanut involves a wide range of molecular mechanisms and metabolic processes.Under waterlogging stress,peanut roots may improve their waterlogging tolerance by enhancing energy metabolism,regulating cell structure components,and regulating stress protein expression.5.The Venn diagram analysis of differentially expressed proteins showed that 156 specific expression proteins related to waterlogging tolerance appeared in the taproot of xianghua 2008.The up-regulated expression of protein Alcohol dehydrogenase,Phosphoglycerate kinase,Stem-specific protein and down-regulated expression of protein Indoleacetic acid amino acid synthetase and Indoleacetic acid amino acid hydrolase were all closely related to the stress resistance of the taproot.8 specific expression proteins related to waterlogging tolerance appeared in the lateral root of xianghua 2008.Among the up-regulated proteins,Tryptophan-trna ligase and Trehalose-phosphate synthase play an important role in the survival and growth of plants in adverse conditions,which are closely related to the waterlogging resistance of the lateral roots of Xianghua 2008.The number of differentially expressed proteins in the taproot was much higher than that in the lateral root,indicating that the relationship between taproot and stain resistance was closer than that in the lateral root.