The Correlation Analysis Of Lead Toxicity Alleviation And Gut Microbiota Regulation By Lactobacillus Plantarum CCFM8661

As a toxic environmental pollutant,lead(Pb)can enter the human body through the food chain.Dietary Pb absorption mainly occurs in the gut tract,and an increasing number of studies have shown that Pb can affect gut microbiota.Lactobacillus plantarum CCFM8661 has been shown to reduce Pb accumulation in tissues and promote Pb excretion.This probiotic can affect intestinal flora and have the potential to alleviate Pb toxicity by regulating gut microbiota.In this study,the effects of L.plantarum CCFM8661 intervention on gut microbiota were investigated in mice exposed to Pb at different doses.Firstly,the changes of gut microbiota were studied after different doses of short-term and long-term Pb exposure-acute Pb exposure and chronic Pb exposure,respectively.Results showed that 6 genera in gut microbiota altered significantly due to acute Pb exposure.With the increase of dose,the relative abundance of Staphylococcus increased linearly,while the relative abundance of Romboutsia and Oscillibacter decreased linearly.Due to chronic Pb exposure,34 genera significantly changed,among which,the relative abundance of Family XIII AD3011 group,Marvinbryantia,Ruminococcus 1,Lachnospiraceae UCG-006,GCA-900066225,Erysipelatoclostridium,[Eubacterium] nodatum group and ASF356 were significantly positively correlated with Pb dose;Lactobacillus had a significant negative correlation with Pb dose.Both acute and chronic Pb exposures have dose-dependent effects on the functions of gut microbiota,according to the results of Picrusts analysis.Therefore,acute and chronic Pb exposures both disturb gut microbiota composition and function,with chronic Pb exposure having a greater effect.Next,the effect of L.plantarum CCFM8661 on gut microbiota in various doses of chronic Pb exposure model was showed that the Pb-induced alterations of Ruminococcaceae UCG-005 and Bilophila could be alleviated to a certain extent.Moreover,the probiotic had a more obvious regulating effect on the altered bacteria at higher dose.As for the gut microbiota functions,the protective effect of CCFM8661 on the 0.5 g/L group was more significant,which could significantly restore the changes of phenylpropionic acid biosynthesis,butyric acid metabolism,propionic acid metabolism,sulfur metabolism and the mutual conversion between pentose and gluconate.After L.plantarum CCFM8661 intervention,the influence of altered gut microbiota on serum and intestinal metabolites in mice was analyzed based on metabolomics.Pb exposure affects purine metabolism in serum of mice,and CCFM8661 also significantly affects it.Lead exposure affects the biosynthesis of aminoyl-t RNA,the biosynthesis of phenylalanine,tyrosine and tryptophan,and the metabolism of phenylalanine which also can be obviously regulated by CCFM8661.The restorative effect of probiotics on serum metabolites was stronger at the dose of 0.5 g/L,while the restorative effect on colonic metabolites was more obvious at the dose of1.0 g/L.Results of Spearman correlation analysis revealed that the effects of Lactobacillus plantarium CCFM8661 on Pb-altered serum metabolites were related with those in the levels of Ruminococcaceae NK4A214 group,Ruminococcaceae UCG-014,[Eubacterium]ventriosum group and Bilophila;In colon,the effects of Lactobacillus plantarium CCFM8661 on Pb-altered metabolites were associated with those in the relative abundance of Bifidobacterium,Coriobacteriaceae UCG-002,Bacteroides,Family XIII AD3011 group,Lachnoclostridium,Lachnospiraceae UCG-006 and Bilophila.Above all,the Pb-induced disorder of gut microbiota disturbance are influenced by the Pb exposure period and Pb exposure dose with changes in the host serum and intestinal metabolite levels.L.plantarum CCFM8661 could regulate the altered bacteria due to Pb and relieve the change of the metabolites in serum and intestinal tract against Pb poisoning.It may be regulating gut microbiota to alleviate the Pb toxicity in the host metabolism.