Research On Cell Culture System Based On Portable Microfluidic Chip

The behavior of cell population determines life activities and directly reflects the physiological and pathological conditions in the body.Compared with traditional experimental methods,living cell experiments can provide more biological information,improve people's understanding of life mechanisms,and promote the development of biomedicine and clinical applications.The study of cell behavior and function relies on environmental conditions such as temperature,humidity and gas provided by the in vitro living cell culture system.However,traditional living cell culture systems generally have shortcomings such as high cost and poor portability.They are not suitable for long-distance transportation of biological samples,which greatly affects the activity of cells and biological tissues,resulting in poor reproducibility of biomedical experiment results.In this research,a cell culture system based on polydimethylsiloxane(PDMS)microfluidic chip was developed to address the above-mentioned problems.The main research contents are summarized as follows:1.The system has a small volume which is 2000 times smaller than conventional cell culture equipment,and can be operated and powered by a Li battery.It is ideal for biological sample transportation and point-of-care detection.Two crucial problems have been solved in the development of the culture system.One is the poor cell activity caused by the chemical environment variation in cell culture cavity,as a result of water evaporation through PDMS nanopore structures.The other is eliminating the negative effect caused by temperature change.2.Based on this cell culture system,this research has achieved long-term culture of primary cells and cell lines.Experiments show that in the portable cell culture system,the cells are in good condition and proliferate in a large amount,reaching the same level as the traditional cell culture system.3.Combining this cell culture system with a high-throughput chip,this thesis studied the toxic effect of the pro-inflammatory factor S100A9 concentration on neural stem cells(NSC).Through screening of more than 5000 drug concentration combinations within one week,this study determined the optimal combination of platelet-derived growth factor(PDGF)and epidermal growth factor(EGF)concentrations to inhibit neuronal toxicity caused by the proinflammatory factor S100A9(EGF: 10?100 ng/ml,PDGF: 0.1?1 ?g/ml).4.Combined with the self-built inverted fluorescence microscopy system and cell culture system,this study analyzed the photo-induced biotoxicity of 3T3 fibroblasts under different optical conditions,and initially revealed the photo-induced biotoxicity effects of conventional fluorescence microscopy imaging systems..This research provides an effective technical solution for the miniaturization and portability of cell culture,and provides a key component for the research and development of POCT high-throughput detection equipment.