| The neurobiological regulation of animals' energy and nutrient metabolism is one of the most fundamental scientific issues in neurobiology and metabolic biology.Its regulatory failure is also the cause of many human neurological and metabolic diseases,such as eating disorders and obesity.In this thesis,I used Drosophila melanogaster as a model to study the effects of the lack of mineral elements in the diet and sleep deprivation on the food intake behavior of fruit flies.We found that the lack of manganese in the diet caused fruit flies to increase their sensitivity to sweet taste and decrease their sensitivity to bitter taste,which phenocopied the state of hungry flies.In contrast,supplementing the supply of manganese to hungry flies partially rescued their sweet and bitter tastes.In addition to dietary conditions,sleep deprivation also had a profound effect on flies' metabolism and behavior.Sleep deprivation of fruit flies by using mechanical oscillation reduced their sensitivity to sweetness and increased their sensitivity to bitterness.We showed that pharmacological manipulations of multiple neurotransmitter systems affected such phenotype and in the future we planned to use chemoconnectome system to explore the genetics and neural circuit mechanisms behind the behavioral effects of metabolic stress such as mineral element deficiency and sleep deprivation.To prepare for a full scale screen,we conducted a quantitative analysis of the main nutrient storage substances,body weight,body length and sleep indicators of the chemoconnectome mutant fruit flies under both fed and starved conditions.Studying how the central nervous system perceives and regulates the behaviors related to energy intake,as well as the causes of perception disorders,will provide us with brand new information for our ultimate understanding of the advanced cognitive functions of higher animals and even human brains,as well as related neurological and metabolic diseases. |
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