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Research On The Role Of Long Non-Coding RNA In Seneca Valley Virus Infection And Seneca Valley Virus Rescue

Posted on:2021-08-20Degree:MasterType:Thesis
Country:ChinaCandidate:M Y ZhuFull Text:PDF
GTID:2480306608954459Subject:Prevention of Veterinary Medicine
Abstract/Summary:PDF Full Text Request
Seneca valley virus(SVV)causes ulcerative changes in the muzzle or hoof wall of pigs,causing huge economic losses to the pig industry.In recent years,Seneca valley virus disease has been sporadic.Although long non-coding RNAs(lncRNAs)have been shown to play an important role in the regulation of RNA and have a profound impact on a variety of biological processes,the role of lncRNAs in the course of SVV infection remains unclear.Therefore,it is necessary to further explore the potential association between lncRNAs and SVV and the viral characteristics of SVVIn order to explore the relationship between host lncRNA and SVV,the expression profiles of lncRNA and mRNA were analyzed for the first time using RNA-seq technology on SVV-infected ST cells.In this study,1332 lncRNAs and 3299 mRNAs were differentially expressed after viral infection,and the function of differentially expressed lncRNA was analyzed by GO and KEGG.In this paper,the lncRNA-miRNA-mRNA ceRNA network was constructed to link lncRNA with miRNA and mRNA that may play an important role in virus-host interaction,providing a basis for better understanding the potential association between host lncRNAs and SVV.In this study,RNA-seq data were identified by qRT-PCR,and lnc-MSTRG.18940.1 was further explored.It was found that SVV could significantly up-regulate the transcription of MSTRG.18940.1 in a dose-dependent manner.Gene silencing of lnc-MSTRG.18940.1 by siRNA and shRNA can significantly inhibit SVV infection.lnc-MSTRG.18940.1 silencing can also inhibit the inflammatory cytokines IL-1??IL-6?IL-8?IL-12 and TNF-?,thus inhibiting the inflammatory response.This study provides a new direction to further explore the regulatory role of lncRNA in the process of SVV infection.The recombinant plasmid of CMV-T7 co-promoter type was established by synthesizing the entire genome of the SVV strain CH-FJ-01 and cloning it to pcDNA3.1.The rescued virus were identified by PCR and sequencing,and the virus growth curve was drawn after the stable virus was passed on the ST cells,which provided a research basis for the later study of SVV virus characteristics and vaccine research.
Keywords/Search Tags:Seneca valley virus, RNA-seq, long non-coding RNA, gene silencing, rescue of virus
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