Effects Of Temperature On Physiologicalactivity And Intestinal Microflora Of Eremias Argus And Takydromus Amurensis

Warming has increased the probability of extinction for many vertebrates,with ectotherms such as reptiles being the most affected.Rising ambient temperatures can be a challenge for temperature-dependent reptiles.It is important to understand the impact of rising temperatures on lizards in the current climate in order to assess their vulnerability to climate change.Therefore,in this paper,Eremias argus and Takydromus amurensis in cold areas with high latitudes are taken as research objects to explore the effects of simulated moderate climate warming on physiological activity and intestinal flora.Research indicates:1.Under the simulated moderate warming,there was no significant difference in snout-vent length(SVL),body mass(BM)and specific growth rate(SGR)between the two groups of E.argus.There were significant differences in SVL,BM and SGR-SVL between the two groups of T.amurensis,and the warming group was significantly higher than the normal temperature group.2.The active body temperature of the two lizards in warming group was significantly higher than that in normal temperature group.During the day,the body temperature of the spotted lizard reached a peak at 12 a.m.,while the body temperature of T.amurensis also peaked at 13 p.m.3.The locomotor performance of the two lizards was significantly different from that of the normal temperature group,and increased with the increase of test temperature.The resting metabolic rate was significantly different between the warm and normal temperature groups of E.argus,which was higher than the warming group.The interaction between body temperature and treatment of T.amurensis has a significant impact on its resting metabolic rate.With the increase of body temperature,the resting metabolic rate under heating conditions is significantly inhibited,and the temperature.Also,Q10(the temperature coefficient)decreases significantly with increasing temperature.4.There is a significant difference in the proportion of intestinal flora between the two lizards' warming group and the normal temperature group,but the core flora is basically the same.The core flora of E.argus is Bacteroidetes,Proteobacteria,Firmicutes and Verrucomicrobia.The core flora of T.amurensis are Proteobacteria,Bacteroidetes,Firmicutes and Fusobacteria.5.After the temperature rises,the contents of Bacteroidaceae and Bacteroides in the intestinal flora of E.argus increased significantly.The contents of Bacteroidetes,Bacteroidales,Bacteroidaceae and Bacteroides in the intestinal flora of T.amurensis increased significantly,while the contents of Enterobacteriales decreased significantly.This study reveals that moderate climate warming may have beneficial effects on lizards in cold areas at high latitudes.It is predicted that reptiles in cold areas may be more suitable for moderate climate warming.The results of intestinal flora also showed that the abundance of pathogenic bacteria in lizard's intestinal tract increased slightly after warming.In the future,it may be necessary to pay more attention to whether the increase of pathogenic bacteria in lizard's intestinal flora after long-term warming will have a negative impact on lizards.