The Role Of THBS1 And KCNT2 In The Proliferation Of Rat Mesenteric Artery Endothelial Under High Salt Environment

BackgroundHigh-salt diet has become one of the three major dietary risk factors.Numerous studies have shown that high salt intake can lead to increased blood pressure,impaired endothelial function,and target organ damage,leading to increased risk of cardiovascular and other chronic diseases.Vascular endothelial cells are one of the main cells that maintain vascular homeostasis,and endothelial cell dysfunction can lead to severe cardiovascular dysfunction.THBS1(Thrombospondin-1)is a potent inhibitor of endogenous physiological and pathological angiogenesis.Studies have shown that it acts on vascular endothelial cells and is of great significance to cardiovascular diseases.Our previous study found that THBS1 plays a key role in the proliferation of rat mesenteric artery endothelial cells in a high-salt environment,and then through transcriptome sequencing technology,we found that high-salt acts on endothelial cell proliferation target gene KCNT2(Potassium sodium-activated channel subfamily t member 2),and finally through RT-q PCR and CCK-8 proliferation experiments to explore the regulatory role of THBS1 and KCNT2 in the proliferation of rat mesenteric artery endothelial cells in a high-salt environment,so as to provide some information for the treatment and prognosis of cardiovascular diseases.new ideas.Purpose(1)To explore the effect and mechanism of THBS1 on the proliferation of mesenteric artery endothelial cells in a high-salt environment.(2)To explore the effect of KCNT2 on the proliferation of mesenteric artery endothelial cells.(3)To explore whether THBS1 and KCNT2 co-regulate the proliferation of mesenteric artery endothelial cells in a high-salt environment.Methods1.The primary cultured rat mesenteric cells were identified as endothelial cells by immunofluorescence assay;2.CCK-8 assay to detect the effect of THBS1 on the proliferation of mesenteric artery endothelial cells under high salt environment;3.Transcriptome sequencing technology found target genes related to the proliferation of mesenteric artery endothelial cells caused by THBS1;4.RT-q PCR experiment to verify the target gene found by sequencing: KCNT2;5.The effect of KCNT2 on the proliferation of mesenteric artery endothelial cells was detected by CCK-8 assay;6.CCK-8 assay to detect whether THBS1 and KCNT2 act together on the proliferation of rat mesenteric artery endothelial cells under high salt environment;ResultsIn this study,we first found that knockdown of THBS1 in a high-salt environment inhibited the proliferation of rat mesenteric artery endothelial cells.The results of transcriptome sequencing indicated that KCNT2 may be a target gene acting on the proliferation of rat mesenteric artery endothelial cells.The expression of KCNT2 in high-salt-cultured endothelial cells was consistent with the sequencing results.Finally,the CCK-8 experiment further found that KCNT2 could affect endothelial cell proliferation,and THBS1 and KCNT2 co-regulated endothelial cell proliferation in high-salt environment.ConclusionKnockdown of THBS1 in rat mesenteric artery endothelial cells under high-salt environment can significantly inhibit endothelial cell proliferation.KCNT2 is an important target of THBS1 to regulate endothelial cell proliferation under high-salt environment.KCNT2 can regulate rat mesenteric artery endothelial cells under high-salt environment.Cell Proliferation.This study provides some new therapeutic ideas for THBS1 and KCNT2 in high-salt-induced cardiovascular disease.