Construction Of PMPC Polymer Brush By Imitation Mussel Adhesion ARGET-ATRP And Its Application

Biofouling is a common problem in the field of biomedicine and oil-water membrane filtration separation.The most effective way is to modify the material with a zwitterionic polymer to enhance the anti-fouling effect.The zwitterionic polymer can be fixed on the surface of the material by atom transfer radical polymerization(ATRP).However,the reaction conditions in the conventional polymerization process are harsh and require a large amount of copper salt as a catalyst,which has potential toxicity problems for biomedical materials.Therefore,this paper establishes a common technical method for constructing polymer brush modified coatings on different material surfaces.poly(2-methacryloyloxyethyl phosphorylcholine)(PMPC)super-hydrophilic coating,poly(Trifluoroctyl methacrylate)(PFOMA)hydrophobiccoating,poly(2-methacryloyloxyethyl phosphorylcholine-b-trifluorooctyl methacrylate)(PMPC/PFOMA)amphiphilic coating was constructed by electron transfer-activated regenerative atom transfer radical polymerization(ARGET-ATRP)under mild conditions in air and copper salt in ppm.The resulted PDA/Si-ATRP/PMPC coating was demonstrated highly biocompatible and bio-fouling resistant.Furthermore,PDA/Si-ATRP/PMPC and PDA/Si-ATRP/PMPC/PFOMA coatings were shown obvious demulsification effect.The main research is as follows:(1)Firstly,The(3-trimethoxy)propyl 2-bromo-2-methylpropionate(Si-ATRP)initiator was covalently bonded to the surface of the material by a polydopamine(PDA)mediated Si-ATRP layer.In the air environment,ppm level of copper salt and excess amount of ascorbic acid aqueous solution,2-methacryloyloxyethyl phosphorylcholine(MPC)monomer was polymerized from the surface of the substrate to form a dense PMPC brush.Explored the best conditions for coating construction.The results of static contact angle(SCA),dynamic contact angle(DCA),X-ray photoelectron spectroscopy(XPS),scanning electron microscopy(SEM),and atomic force microscopy(AFM)confirmed that PDA/Si-ATRP/PMPC super hydrophilic polymer brush was successfully constructed by this polymerization method.(2)Protein and plasma adsorption,platelet adhesion,whole blood adhesion,cell adhesion experiment results showed excellent biocompatibility and anti-biofouling property of the PDA/Si-ATRP/PMPC coating surface.BSA,Fg and plasma adsorption amounts are all lower than 1.9 ng/cm~2,which is much better than the requirement of?5 ng/cm~2 for excellent anti-biofouling surfaces.(3)poly(2-(perfluorohexyl)ethyl methacrylate)(PFOMA),Poly(2-methacryloyloxyethyl phosphorylcholine-b-trifluorooctyl methacrylate)(PMPC/PFOMA)coatings were constructed by ARGET-ATRP on PDA/Si-ATRP coated glass substrates.By testing static contact angle(SCA),X-ray photoelectron spectroscopy(XPS),scanning electron microscopy(SEM),atomic force microscopy(AFM),the results onfirmed the successful construction of the coatings.The results of an oil-in-water emulsion interaction with the bare glass,PDA/Si-ATRP/PMPC,PDA/Si-ATRP/PFOMA,PDA/Si-ATRP/PMPC/PFOMA coatings demonstrated that PDA/Si-ATRP/PMPC and PDA/Si-ATRP/PMPC/PFOMA coatings had obvious demulsification effect.