Research On Inosinic Acid Detection Method Based On Enzyme Biosensor

The meat is popular among the public due to its rich nutrition and delicious taste.The nucleotides are mainly contributed to the umami substances.Especially IMP has a great effect on umami of meat during the cooking process.The content and existence of IMP is not only related to the deliciousness of meat products,but also considered as an important index to evaluate the freshness of meat products.This paper explored and prepared an enzyme biosensor to detect IMP in meat.This study was aimed at selecting an enzyme biosensor with high specificity,low cost and fast analysis speed,and an IMP detection method that can directly reflect the detection results was explored and designed.By analyzing the size and regularity of the reaction signal of the enzyme biosensor,a highly sensitive and intuitive method for quantitative detection of IMP was also developed.The specific research contents and results of this paper are as follows:1.Based on the catalytic activity and conductivity of Au@Pt nanoflowers,a novel enzyme-free sensor was assembled and prepared for sensitive and rapid analysis of the content of IMP enzymatic by-product H₂O₂.The materials were characterized by emission scanning electron microscopy and X-ray diffraction.The results showed that the dandelion-like nanoflowers were formed with Au particles centered and surrounded by Pt particles.Au@Pt nanoflowers were used as a nano-enzyme to catalyze the oxidation of H₂O₂,and the electrochemical behavior of H₂O₂on the modified electrode was studied.The results showed that the sensor based on Au@Pt nanoflowers had wide linearity,high sensitivity,anti-interference,and reproducibility The cyclic voltammetry curve and time current curve of H₂O₂on the modified electrode were also studied.The results showed that the sensor loaded with Au@Pt nanoflower had a good linear range(0.03?1100?mol/L)and high sensitivity(detection limitup to 0.02?mol/L),Compared with the traditional titration method,the new enzyme-free sensor can quickly and accurately detect the H₂O₂content in a complex environment.2.The study utilized the conductivity of MXene materials and the catalytic ability of Au@Pt nanoflowers to assemble and prepare enzyme biosensors for ultrasensitive and rapid detection of IMP.The results showed that the MXene-loaded Au@Pt nanoflower-based enzyme biosensor had a good linear relationship in the range of 0.04?17g/L,with a correlation coefficient of 0.996 and a detection limit of 2.73 ng/m L.3.The biosensors modified by MoFs composite nanomaterials were used to detect IMP.MoFs nanocomposites were prepared on the surface of the electrode in the design of enzyme biosensors.They have high specific surface area,biocompatibility and porosity,and enhancethe ability of loading enzymes.MoFs has a great catalytic effect similar to horseradish peroxidase,which can catalytically decompose H₂O₂produced by the process of enzymatic hydrolysis,further expanding the electrical signal to achieve the desired detection limit.The new enzyme biosensor was more sensitive,efficient,and quantitative to detect IMP.The concentration range of IMP was 0.05?0.3 g/L,and the linear regression equation was:Y=4.271 X+8.044(R²=0.997).The detection limit was 0.03?mol/L,which could be used for qualitative and quantitative detection of IMP in meat products.This study validated the feasibility of enzyme biosensors for IMP detection,and different enzyme sensors were conducted using nanomaterials to assist signal amplification.The results showed that the enzyme sensor using the simulated enzyme nanomaterials to assist the target cycle amplification had lower detection limit and simpler operation.