Preparation,characterization And Morphological Control Of A Multi-responsive Boronate-based Nanostructure

To enhance the specific responsiveness of nano carriers in the tumor,two kinds of reactive oxygen species(ROS)responsive bis-phenylboronic monomers were designed to assemble with pentaerythritol.With dynamic covalent bond properties of boronate ester and the p-? stack effect of B-benzene,these monomers can form nanoparticles by poly-condensation.Catechol-linked polyethylene glycol(m PEG-CA)was added to improve the stability in water and change the size of these particles.When the nanoparticles are uptaken in tumor cells,boronate ester bond can hydrolyze under acid condition and phenylboronic acid monomer also can be broken by reactive oxygen species.So that,these nanoparticles will degrade rapidly and the loaded drugs are released into tumor cells.During this work,nuclear magnetic resonance data were used to prove that catechol was successfully attached to m PEG,and bis-phenylboronic monomers were successfully prepared with high purity.Dynamic light scattering data and TEM imagines show that two monomers can both form particles in methyl alcohol with pentaerythritol,but hard to remain stable in water.When added with m PEG-CA,the size became smaller and the particles can be stable in water.Finally,the acid sensitivity and oxidative sensitivity of the nanoparticles were confirmed by simulating the acid concentration and the concentration of reactive oxygen species in the microenvironment of the tumor,which provided the basis for further in vivo experiments.This new material based on double sensitivities and rapid response provides a new type of nano-carrier for the delivery of anti-tumor drugs,which provides a new idea for nano-carrier.