Application Of Nano-Composite Materials Based On FePt Nano-Particles In Multi-Functional Treatment Of Tumors

Research Background:The widespread development of cancer has become the leading cause of human death.Surgery,radiation therapy,and chemotherapy are the three main methods of treating cancer,but surgery is very traumatic.Removal of the primary lesion may not necessarily prevent tumor metastasis;complications such as radiation dermatitis and the resistance of normal tissues around the tumor Dose limits the effectiveness of radiation therapy;the toxicity of drugs produced by non-specific chemotherapy can cause great side effects on the human body.At the same time,chemotherapy resistance and radiation tolerance caused by hypoxic tumor cells have brought great obstacles to tumor treatment.Therefore,in recent years,the combination of multiple therapies for the treatment of tumors has continuously entered people's field of vision.At the same time,the emerging nanotechnology in the field of biomedicine has brought new directions to tumor treatment in recent years.Published studies show that nanomaterials can be used in drug carriers ? radiation sensitization ? tumor imaging ? specific drug targeting ? photothermal conversion ? magnetic thermal response.FePt nanoparticles have attracted our attention due to their advantages such as simple synthesis,uniform particle size,good radiosensitization ability,and excellent biocompatibility.They are now used in MRI / CT dual-mode imaging,drug delivery,chemotherapy,and radiosensitization Aspects are applied.The multifunctional application of nanocomposites with FePt nanoparticles as the main body in tumor treatment has not been reported.Research purposes:To investigate the effect of MGO / FU-MI nanocomposites(MGO / FU-MI NCs)with FePt nanoparticles as the main component in tumor chemotherapy ?radiochemotherapy combination and the feasibility of FePt @ GO nanocomposites(FePt @ GO NCs)in tumor photothermal treatment.Research methods:(1)MGO / FU-MI NCs with different concentrations were co-cultured with MC-38 and B16 cells,and the cell survival rate was measured with the CCK-8 kit,and theapoptosis rate,cell cycle,and cells were measured by flow cytometry.Internal reactive oxygen species(ROS)detection validated some of the toxicological mechanisms of nanocomposites.(2)The MGO / FU-MI NCs was used in combination with 2Gy radiation.The CCK-8 kit was used to detect the cell survival rate and the flow cytometry was used to detect its apoptotic rate.(3)Construct a mouse abdominal cavity tumor model,and inject MGO / FU-MI NCs of different concentrations into the abdominal cavity of mice in three times to observe the effects of nanocomposites on tumor growth and mouse survival.(4)Combine the MGO / FU-MI NCs with 5Gy radiation to observe its effect on tumor growth and mouse survival.(5)Put different concentrations of FePt @ GO NCs under different power near-infrared light sources,observe and record their temperature rise and their light-to-heat conversion stability.(6)Different concentrations of FePt @ GO NCs were co-cultured with MC-38 cells and irradiated with 1W near-infrared light.The CCK-8 kit was used to detect its cell survival rate,and the flow cytometry was used to detect its apoptotic rate.(7)Construct a mouse subcutaneous implant tumor model,inject FePt @ GO NCs of different concentrations into the tumor body three times,and irradiate with 1W near-infrared light to observe the tumor growth of the mouse.Research results:(1)MGO / FU-MI NCs have obvious toxicity to two kinds of cells in vitro,can cause S-phase arrest of cells,stimulate the increase of intracellular reactive oxygen species,and have a very obvious inhibitory effect on cell proliferation.(2)When MGO / FU-MI NCs are used in combination with radiation,the apoptosis rate of tumor cells is significantly increased compared to a single treatment.(3)MGO / FU-MI NCs can suppress tumor proliferation in mice and prolong mouse survival.(4)When MGO / FU-MI NCs is used in combination with radiation,compared with a single treatment,it can more effectively suppress the tumor growth in mice and prolong the survival period of mice.(5)FePt @ GO NCs with different concentrations are significantly heated under the irradiation of near-infrared light with different powers,and the higher the concentration / power,the faster the temperature rise,and FePt @ GO NCs have good light-heat conversion stability.Sex.(6)When FePt @ GO NCs is used in combination with near-infrared light,it can significantly increase the apoptosis rate of tumor cells.(7)FePt @ GO NCs combined with near-infrared light can effectively inhibit tumor proliferation in mice.Conclusion:MGO / FU-MI NCs have achieved very significant results in the combined treatment of tumor chemotherapy and radiochemotherapy.Especially in the combination therapy of radiotherapy and chemotherapy,they have achieved more excellent results than single treatment,which has great clinical significance.And for the first time,we used FePt @ GO NCs for tumor photothermal therapy.FePt @ GO NCs showed good photothermal response stability and significant therapeutic effects in vivo and in vitro during photothermal therapy.The main body of nano-composite materials plays a very important role in the research of multifunctional tumor therapy.