Preparation Of Lanthanide Metal Coordination Polymer And Its Application In Biomarkers

Alzheimer's disease(AD)is a neurodegenerative disease in which patients have memory decline,slow mobility,and loss of autonomous mobility.The disease mostly occurs in the elderly.With the aging of the world population,people have realized that a simple and sensitive method can be developed to achieve the purpose of early diagnosis and prevention of AD and follow-up treatment in the later period to reduce the incidence of AD.It is of great significance.Lanthanide metal coordination polymers have attracted people's attention in recent years due to large Stokes shift,long fluorescence lifetime,inherently narrow f-f emission band and excellent quantum efficiency,and are widely used in various fields.Among them,the application of detecting biomarkers has become the focus of the biological and medical circles,especially in cancer and tumor applications.In this paper,three different lanthanide metal coordination polymers were designed and synthesized,which were successfully used for white light emission,protein kinase A detection,and? amyloid detection.(1)In the past,the synthesis of lanthanide metal-organic coordination polymers(LMOCP)used to emit white light was carried out at high temperatures.At the same time,the organic ligands in these LMOCPs are toxic.Therefore,non-toxic reagents are used for green synthesis performance.Good white light materials are necessary.We mainly prepare white light lanthanide metal-organic coordination polymers through a green synthesis method,which is carried out in a pure aqueous solution at room temperature and does not use toxic solvents and reagents.This LMOCP,expressed as AMP/Ln-CIP,is composed of Ln3+{Ln=Tb(1),Eu(2)and Gd(3)},AMP(adenosine monophosphate)water-soluble biomolecules and CIP(ciproxazole)Star)composed of non-toxic antenna ligands.The Tb(1),Eu(2)and Gd(3)complexes in AMP/Ln-CIP emit strong green,red and blue light,respectively.By carefully adjusting the doping molar ratio of the three lanthanide ions {Ln=Tb:Eu:Gd:=0.1:0.9:99.0} in a frame,white light emission can indeed be achieved.In addition,the fluorescence lifetime and quantum yield of AMP/Ln-CIP are 4.36 ms and 63.5%,respectively.(2)In this study,a novel luminescence detection method for protein kinase A(PKA)activity was designed using lanthanide metal organic coordination polymer nanoparticles as probes.Tb/ATP-Zn itself is self-assembled by adenosine triphosphate(ATP),Zn2+ and Tb3+,and does not emit light.PKA can catalyze the conversion of ATP to ADP in Tb/ATP-Zn nanoparticles,thereby effectively sensitizing the luminescence of Tb3+.Based on this mechanism,Tb/ATP-Zn can achieve simple luminescence "on" sensing of PKA activity without using exogenous ATP and substrate peptides.Optimize each experimental condition.When the optimal concentrations of ATP,Zn2+and Tb3+are 0.3 mM,0.2 mM,0.4 mM,and pH=7.4,the fluorescence intensity and PKA concentration of Tb/ATP-Zn probe at 545 nm It shows a good linear relationship in the concentration range of 0.1-2.0 U/?L.The detection limit of this method is as low as 0.001 U/?L,and it also has the characteristics of high selectivity,long-term stability,rapid response and convenient operation.In addition,Tb/ATP-Zn was successfully used to monitor the activity of PKA in cell lysates.Therefore,Tb/ATP-Zn is expected to become a powerful tool for studying protein kinase-related biomolecules.(3)?-amyloid protein(A?)is composed of peptide fragments of 39-43 amino acids.The misfolding of A? is one of the pathological mechanisms of Alzheimer's disease that is easily accepted and recognized by people.It is necessary to detect Alzheimer's The A? in patients with Alzheimer's disease has extraordinary significance for the early diagnosis and treatment of Alzheimer's disease patients.Our research is to prepare lanthanide metal-organic coordination polymer(LMOCP)Tb/GMP-CR,(GMP=guanosine-5'-monophosphate guanine monophosphate)through post-synthesis modification(PSM)method at room temperature.Sodium phosphate,CR=Congo red,Congo red)a fluorescence-enhanced analysis platform to detect A?1-40.In the Tb/GMP-CR lanthanide metal organic complex,Tb3+is the metal ion luminescence center,GMP is the organic bridging ligand,GMP can effectively sensitize the luminescence of terbium ions(Tb3+),making Tb-GMP emit very strong fluorescence.However,when the PSM method is used to introduce CR into Tb-GMP,the Tb/GMP-CR formed may emit extremely weak fluorescence due to resonance energy transfer.Happily,when A?1-40 is added then it induces to emit fluorescence with almost the same intensity as the original Tb-GMP complex,which is mainly due to the interaction between A?1-40 and CR.Combining the luminescence characteristics lanthanide ions and the specific binding force of CR and A?1-40,we successfully prepared a fluorescence-enhanced lanthanide coordination polymer analysis platform to achieve highly sensitive detection of A?1-40.