Comparative Study On The Difference Of Sugar Accumulation In The Fruits Of Different Wine Grape Varieties In The Eastern Foot Of Helan Mountain

In order to understand the difference of sugar accumulation of different varieties under the geographical environment at the eastern foot of Helan mountain,16 wine grape varieties in Vitis Vinifera×Vitis Amurensis,Vitis Vinifera and V.vinifera × V.labrusca were used as research objects.The soluble sugar in fruit was determined by HPLC method,and the activities of key enzymes of sucrose metabolism were determined.Transcriptome sequencing and fluorescence quantitative PCR were used to explore the expression of differential genes in sugar metabolism.The results show that:1.The fructose content of Vitis Vinifera × Vitis Amurensis(red)in young fruit stage,fruit expansion period,complete color conversion period and mature stage was significantly higher than that of Vitis Vinifera(red),which increased by 68.72%,16.18%,19.52%and 19.20%respectively.The glucose content of wine grape of Vitis Vinifera × Vitis Amurensis(red)species in complete color conversion stage and mature stage was 16.70%and 16.77%higher than that of Vitis Vinifera(red)species,respectively,and the sucrose content was 28.70%and 41.83%higher than that of Vitis Vinifera(ved)species,respectively,and the difference was significant.2.From fruit expansion stage to ripening stage,the Al activity of Vitis Vinifera X Vitis Amurensis(red)species was 18.72%,15.56%,32.27%and 14.83%higher than that of Vitis Vinifera(red)species,respectively,and the difference was significant.During the fruit expansion period and at the initial stage of color conversion,the NI activity of Vitis Vinifera ×Vitis Amurensis(red)species was significantly higher than that of Vitis Vinifera(red)species,which increased by 31.61%and 24.53%,respectively.The SS-c activity of Vitis Vinifera(red and middle maturity)species was significantly higher than that of Vitis Vinifera×Vitis Amurensis(red)before fruit color conversion,and the SS-c activity of Vitis Vinifera(red and late ripening)species was higher after color conversion.Correlation analysis showed that SS-c activity of Vitis Vinifera(red and middle maturity)species was significantly positively correlated with soluble solids,and significantly positively correlated with total sugar,reducing sugar and fructose,while SS-c activity of Vitis Vinifera(Ted and late ripening)species was positively correlated with glucose and sucrose.In young fruit stage and fruit expansion stage,the SS-s activity of Vitis Vinifera × Vitis Amurensis(red)was higher than that of Vitis Vinifera(red)species by 13.16%and 144.50 per cent,respectively.The SPS activity of Vitis Vinifera × Vitis Amurensis(red)species was higher than that of Vitis Vinifera(red)species at 20-95 days after anthesis,and the SPS activity of Vitis Vinifera × Vitis Amurensis(red)species was significantly higher than that of other species at 70-95 days after anthesis,which was consistent with the high sucrose content of Vinifera X Vitis Amurensis(red)species.3.Transcriptome data showed that the number of differentially expressed genes in 45-95 days after anthesis was greater than that in 95-120 days after anthesis,and most of the differentially expressed genes were down-regulated,and the number of differentially expressed genes in‘Cabernet Sauvignon'was the most(6390).The number of significant differentially expressed genes in 'Pinot Noir' reached 4908 at 95-120 days after anthesis.The differential genes among different species are mainly enriched in metabolic pathways such as carbon metabolism,photosynthesis and sugar accumulation.There are 67 differential genes related to glucose metabolism,and there are 40,35,36 and 44 significant differentially expressed genes in 'Beihong',‘Cabernet Sauvignon','Pinot Noir' and‘Marselan'respectively,mainly including sugar transporter,sucrose phosphate synthase,sucrose synthase,plant neutral invertase,plant invertase and so on.4.During the harvest period,the 100-grain weight of Vinifera× Vitis Amurensis(red)wine grapes was significantly higher than that of other varieties,and the 100-grain weight of Vitis Vinifera(red and late ripen)was the lowest;the content of titratable acid and anthocyanins of Vinifera X Vitis Amurensis(red)was significantly higher than that of other varieties,it is 15.03%and 74.21%higher than the Vitis Vinifera species(red).The content of tannins and total phenol in Vinifera X Vitis Amurensis(red and mid-ripe)are significantly higher than other species.The 100 grain weight,titratable acid,tannins,and total phenols in Vitis Vinifera{white)wine grapes at harvest were 7.47%,44.82%,44.20%,12.14%higher than those of V.vinifera×V.labrusca species,respectively.However,the content of soluble sugar except glucose in V.vinifera X Vlabrusca species was significantly higher than that in Vitis Vinifera(white)species.5.The up-regulated expression of VvcwINV,VvSS,VvSPS and VvSUC11 genes in the late fruit development stage of Vinifera × Vitis Amurensis(red)wine grape was 34.39%,25%,70.93%and 4.93%higher than that of Vitis Vinifera(ved)species,respectively,so it increased the activities of enzymes related to sugar metabolism,increased the contents of sucrose,glucose and fructose,and finally accumulated more sugar in the fruit than Vitis Vinifera(red)species.The increase of sugar content in the fruit also led to the increase of anthocyanin content.The fruit quality was improved.