Study On The Co-assembly Behavior And Performance Of Collagen/Fibroin Blend System In Vitro

Collagen,as a natural biological molecule,is widely distributed in animals,not only as a critical component of the extracellular matrix,but also in the construction of organ tissue,the function of the body and the regulation of cell behavior.Because collagen has good biocompatibility,non-toxicity,degradable and other unique energy,it is widely used in medical materials.The silk protein extracted from the silkworm is another widely derived natural large molecule,and also has excellent biocompatibility,mechanical energy,controllable degradation,and gradually become a mainstream biological material.The cellular interaction and behavior control ability of collagen is strong,but the thermal stability is poor,the performance of biomechanical and the degradation of the degradation are weak,while the fiber is high,the heat stability is high,the degradation is good,and it has good flexibility and mechanical mechanical properties,and the two kinds of biomass are good complementarity in performance.In this study,the analysis of the interaction between collagen and silk protein,the interaction of the two kinds of biological molecules,and the structural energy of the two kinds of molecular interactions,the influence factors and the structure of the hybrid gel products,provided new ideas for the preparation of composite biomaterials.Using Na₂CO₃degum method,the process of removal,soluble silk,dialysis and other process extraction,purification of silk protein water solution,and the analysis of the molecular structure of the sds-pageis by the infrared spectrum,the infrared light spectrum,the amino acid composition analysis.The results show that the preparation of the silk protein in the laboratory is a combination of amino acids,the characteristics of spectroscopy and the unique electrophoresis strip,which is a protein complex with a variety of molecular weight structure.In this paper,the molecular dynamic behavior of two large molecules(assembly),which is used in the synthesis of the molecular dynamics behavior of the two large molecules,is used in the synthesis of the molecular dynamics behavior of the two large molecules.The experimental results show that the coexistence of silk protein can accelerate the assembly speed of collagen,but it is not involved in the assembly.In the same condition,when the assembly time is extended to 2dds,the common assembly of the silk protein and collagen can be observed.Using scanning electron microscopy and transmission electron microscope to observe the microstructure of the assembled products,the samples showed that the samples were shown in the same time that the thick collagen fibers and the fine and random silk protein fibers were present,confirming the collaborative assembly behavior of the silk protein and collagen.By the experiment of fluorescent markers siltin protein,the existence of siltin protein is still found in the products of the synthesis of the silk protein and collagen,and the synthesis of the synthesis of the silk protein and collagen is verified by the molecular level.The product of the gel products of silk protein and collagen was used to study the product of silk protein and collagen,and the performance analysis of the system was carried out.The flow variability of the gel products showed that the viscoelasticity of the combined gel was significantly enhanced than that of the collagen and the silk protein,and the viscoelasticity was enhanced with the increase in the proportion of the silk protein.The thermal stability analysis showed that the combination of collagen and silk protein was only one of the absorption peaks,and between the two absorption peaks of the two of the two,the results showed that the collagen and the silk protein were assembled and formed a new gel product,which increased the heat stability of the material.The results show that the enzyme degradation of the frozen dry sponge is greatly improved,the stability of water resistance is improved,and the mechanical properties and mechanical energy are also increased.This study systematically studied the interaction of silprotein and collagen,and explored the conditions of the two kinds of biomolecules to build the material in the molecular micro level,and the correlation between the co-assembly products was carried out,and the improvement of the composite gel was confirmed in the aspects of stability and mechanical properties.This study has good reference for the design of collagen,silk protein composite materials and new product preparation.