Study On The Functional Food Of Deer Bone-Epimedium Tablets In Increasing Bone Density

Aimed at people suffering from osteoporosis,a Deer Bone Epimedium Tablet with the effect of increasing bone density has been developed.Deer Bone Epimedium Tablets are guided by the theory of traditional Chinese medicine,using Chinese medicines for invigorating the kidney,tonifying the kidney,strengthening the bones and muscles(deer bone,epimedium,rhizoma drynaria)as the foundation,combined with other bone nutritional supplements(glucosamine,calcium carbonate and sulfuric acid)Chondroitin),which can treat both the symptoms and the root cause,promote the absorption and digestion of calcium in the body,regulate the balance of bone formation and osteogenesis in the body,and achieve the purpose of increasing bone density and improving osteoporosis.This study investigates and analyzes the five aspects of formula rationality research,preparation process research,iconic ingredient research,safety evaluation and functional evaluation,and provides technical support for the development and application of Deer Bone Epimedium Tablets health food.Study on the rationality of the formula:consult and analyze the recommended dosage of each raw material in the"Chinese Pharmacopoeia"and a large number of related documents,combined with the theory of Chinese medicine compatibility,determine the daily dosage of this compatible raw material:epimedium 3 g,drynaria 3 g,amino sugar 1 g,deer bone powder 0.5 g,Chondroitin sulfate 1 g,calcium carbonate 1 g.The experiment set up Chinese medicine group(deer bone,epimedium,drynaria fortunei),nutrient group(glucosamine,chondroitin sulfate,calcium carbonate),and compound group(deer bone,epimedium,drynaria fortunei,amino sugar,cartilage sulfate).Calcium carbonate),a positive drug group and a sham operation group were set up.The osteoporosis rats caused by ovariectomized rats were taken as the research object.By giving each group of drugs,the femoral bone density and bone calcium content of each group were measured and the femoral Pathological examination.It is determined that the compound group has the effect of increasing bone density and improving osteoporosis,and the compound group is more effective than the single use of traditional Chinese medicine or nutrition.In this part of the experiment,the formula was screened through efficacy and the final compatibility was determined to be Epimedium,Drynariae,Deer Bone Powder,Glucosamine,Chondroitin Sulfate and Calcium Carbonate.Preparation and production process research:The L~9(3~4)orthogonal test was used to investigate the amount of solvent,extraction time,and extraction times,and the optimal extraction process for Epimedium and Rhizoma Drynariae was optimized,and the dosage form specifications,excipients,fillers,wetting agents,and Lubricants,etc.,determine the preparation process,and scale up the pilot scale to verify the suitability and rationality of the extraction process and the preparation process.The extraction process and preparation process are as follows:pre-soaking for 30 minutes,decocting with 12 times the amount of water for 3 times,each time for 1.5 hours.The filtrate is concentrated under reduced pressure to a thick paste with a density of 1.25 to 1.30(measured at 60?),dried under reduced pressure(-0.06 to?-0.10 MPa,50?70?)to dryness,crushed,and passed through an 80-mesh sieve.Add deer bone meal(irradiated),amino sugar,chondroitin sulfate and calcium carbonate,mix well with dextrin,prepare soft material with 80%ethanol,granulate with 18 mesh,dry at 60?(moisture content<5%),16 mesh finishing particles,and magnesium stearate is added,mixed evenly,and pressed into tablets(tablet weight 0.85g).Research on the iconic ingredients:After the Chinese Pharmacopoeia and a large number of documents,it is determined that the iconic ingredients of Deer Bone Epimedium Tablets are icariin,glycosaminoglycans and chondroitin sulfate.The experimental establishment of icariin,glycosaminoglycans and chondroitin sulfate HPLC detection methods and methodology.The linear relationship is good,and the specificity,stability,repeatability,precision and recovery rate are all well investigated.It shows that the detection methods of icariin,amino sugar and chondroitin sulfate are stable and feasible and can be used as effective methods for the detection of marker components in Deer Bone Epimedium Tablet.Safety evaluation:Taking Deer Bone Epimedium Tablets as the test substance,the acute toxicity test and 30-day normal toxin feeding investigation have been carried out.According to the acute toxicity experiment in mice,it can be seen that the deer bone Epimedium Tablets were administered intragastrically for 14 days,and the mice did not die,the weight gain was normal,and the general anatomy was not abnormal.The oral acute toxicity tolerance is greater than 48 g/kg·bw,and the test substance is non-toxic.After30-day feeding test of Deer Bone Epimedium Tablets,it was observed that there was no abnormality in general conditions;the weight gain,appetite,food utilization rate of male rats,hematology indexes and blood biochemical indexes of rats were not abnormally affected;gross anatomy and internal organs There were no significant lesions in the absolute weight and relative weight of organs(organ/body ratio),and histopathological observations.The above experiments show that Deer Bone Epimedium Tablets are non-toxic productsFunctional evaluation:SD rats fed with low-calcium diet were used as the research object,and Deer Bone Epimedium Tablets were given for a long time to study the effect of Deer Bone Epimedium Tablets on the increase of bone mineral density in rats.The growth and development of rats did not have any effect;compared with the low-calcium group,the bone density,femoral dry weight and bone calcium content of the high-calcium group,the middle-dose group and the high-dose group increased,which was significant.The bone histopathological observation results Significant.It can be judged that Deer Bone Epimedium Tablets help increase bone density and improve osteoporosis.