Regulatory Synthesis And Properties Of Hydroxyapatite Materials

In biomedical materials,hydroxyapatite has unique biological activity and excellent biocompatibility because of its chemical composition similar to that of bone and teeth in nature.It has a good prospect in clinical application.Therefore,it has been a research focus on large-scale preparation of hydroxyapatite with excellent biological properties and unique surface structure.In this paper,by controlling the process parameters such as temperature,time,and additives in the synthesis process,traditional hydrothermal methods and microwave hydrothermal methods were used to prepare nano-hydroxyapatite crystals of different sizes and morp Hologies,and explore the most The best preparation process,the effect of process conditions on the crystal and related mechanisms were studied,and the obtained samples were tested for cytotoxicity to explore the effects of cells of different sizes of nano-crystalline grains.The main contents and results are as follows:Hydroxyapatite was prepared under hydrothermal conditions,and the control and influence of temperature,time,p H and additives on the material preparation process were studied by XRD,FTIR and TEM testing methods.The results show that as the temperature rises and the heating time increases,the crystallinity of the nanohydroxyapatite crystals increases and the size becomes larger.Considering the growth trend of crystallinity of crystals and energy saving considerations,heating for 12 h under hydrothermal conditions at 160°C is a better preparation process condition.Under these conditions,higher p H promoted the growth of the crystal along the c-axis.With the increase of p H,the nano-hydroxyapatite grains changed from short rods to long rods.Study the effect of glycine and arginine on the crystal growth process.Arginine with guanidyl group on the side chain has a stronger inhibitory effect on the crystal growth.The temperature parameters of the hydrothermal method were used to heat the precursors under microwave conditions,and the obtained materials were characterized.The results showed that the crystallinity of the samples was approximated by the microwave hydrothermal method for 30 min and the traditional hydrothermal method for 3 h Equivalent.Therefore,the microwave hydrothermal method can shorten the preparation cycle,reduce costs and save energy consumption,and quickly and effectively prepare mesoporous nano-hydroxyapatite powder materials.Compare the effects of different concentrations of glycine and serine on the crystals.As the concentration increases,the specific surface area of the sample increases,and the size and crystallinity become smaller.Compared with glycine,serine at the same concentration has stronger inhibition of crystals,which is attributed to the effect of hydroxyl groups on the serine side chain.osteoblast toxicity test was performed on all the materials prepared.The results measured by the CCK-8 method showed that nano-hydroxyapatite inhibited cell growth to a certain extent.When the concentration was 50 ?g / ml,compared with the negative blank control group,each group had no obvious toxicity.Comparing nanoparticles with different particle sizes,within a certain particle size range(140 nm-53 nm),as the average particle size decreases,the activity of osteoblasts gradually increases,and the cell compatibility of smaller materials is more excellent.Materials of different sizes prepared using both methods are expected to be applied to biomedical materials.