| Electrochemiluminescence(ECL)as a new detection technology has received more and more attention,through the novel design of the sensor,this method can achieve high selectivity for the detection.In recent years,it has aroused the interest of researchers in the field of analysis and detection,and has been widely used in the environment,food,and medicine,etc.Based on the metal organic framework(MOF)loaded with different luminous bodies,the synergistic effect was used to amplify the signal.carbon-doped copper-based metal organic framework(CDs-HKUST-1),polyethyleneimine(PEI)modified perylene derivatives(PTC-PEI),amino acid-modified perylene derivatives(PTC-Cys)and copper-based metal organic framework doped with gold nanoparticles(Au-HKUST-1)were sequentially prepared,respectively,which were constructed the electrochemiluminescence sensor for the detection of catechol(CC),ractopamine(RAC)and kanamycin(KAN).The specific research content is as follows:1.CDs-HKUST-1 complex modified glassy carbon electrode was designed to detect catechol in tea samples by electrochemiluminescence sensorWe used a simple one-pot hydrothermal synthesis method and controlled the ratio of raw materials to prepare a compound with excellent performance(CDs-HKUST-1)as a light-emitting body.The sensor based on CDs-HKUST-1 had a good ECL response for detecting CC.Under the optimal conditions,the linear range of the sensor for CC detection is5.0×10-9 mol/L?2.5×10-5 mol/L,and the detection limit is 3.8×10-9 mol/L(S/N=3),the method is simple to operate,fast in response and good stability.At the same time,this work broadens the application of HKUST-1 in ECL research.2.HKUST-1/PTC-PEI complex modified glassy carbon electrode was designed as an electrochemical aptasensor for detecting ractopamine in porkIn this work,perylene derivative(PTC-PEI)with good film-forming effect was used as ECL emitter,HKUST-1 with large specific surface area and excellent adsorption performance,it not only improves the ECL efficiency of PTC-PEI through the synergistic effect,but also improves the interface stability of the sensor through the combination of amide bonds.Under the best experimental conditions,the linear range of the sensor for RAC detection is 1.0×10-12 mol/L?1.0×10-6 mol/L,and the detection limit is 6.17×10-13 mol/L(S/N=3),the designed aptasensor can show better sensitivity,stability and specificity.3.Au-HKUST-1/PTC-Cys complex modified glassy carbon electrode was designed for kanamycin detection in milkIn this work,Au-HKUST-1 and PTC-Cys were combined to modify the glassy carbon electrode,and the DNA sequence of kanamycin was introduced to form an Apt/Au-HKUST-1/PTC-Cys composite,HKUST-1,doping gold(Au)nanoparticles,the electron transmission rate was enhanced,and the luminous efficiency of the luminous body can be improved.Under the action of the co-reactant potassium persulfate(K2S2O8),KAN can significantly enhance the ECL strength of Apt/Au-HKUST-1/PTC-Cys/GCE.Under optimal conditions,the enhanced ECL intensity has a good linear relationship with the logarithm of the kanamycin concentration.The linear range of the sensor for kanamycin detection is1.0×10-13 mol/L?1.0×10-8 mol/L,the detection limit is 4.2×10-14 mol/L(S/N=3). |
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