Synthesis Of Luminol Fluorescent Nanomaterials And Detection Of Biomolecules

Fluorescent nanomaterials based on aggregation induced luminescence are characterized by simple synthesis,stable optical properties,high quantum yield and low toxicity.Therefore,they have been widely used in cell imaging,environmental detection,chemical sensing,biological sensing,food detection,disease diagnosis and other aspects.This article mainly studies the synthesis of fluorescent nanomaterials based on aggregation induced luminescence(Luminol-Tb fluorescent nanomaterials,Luminol-Al fluorescent nanomaterials)and their detection of uric acid,alkaline phosphatase and glutathione,including the following three aspects:1.Using the coordination between luminol and terbium ions(Tb³⁺),luminol-Tb fluorescent nanomaterials are synthesized.The fluorescence intensity of the luminol-Tb fluorescent nanomaterial is nearly 40 times stronger than that of luminol,and its maximum excitation wavelength is 290 nm and the maximum emission wavelength is430 nm.Uric acid can quench the fluorescence of luminol-Tb fluorescent nanomaterial by photo-induced electron transfer.Therefore,a highly sensitive fluorescence quenching analysis method for the detection of aluminum ions is established,with the detection range of 0.1-50?M and the detection limit of 28 n M.Compared with some common metal ions,glucose,ascorbic acid,dopamine,creatinine,urea and cysteine,the fluorescence signal of luminol-Tb fluorescent nanomaterials is more affected by uric acid,indicating that this method has high selectivity in detecting uric acid.In addition,the recovery methods of standard additions are used to investigate the accuracy of the detection method and the feasibility of detecting uric acid in actual serum samples.5?M,10?M and 30?M uric acid are added to the serum samples,and the recoveries range from 97.50%to101.30%.It indicates that luminol-Tb fluorescent nanomaterials have high accuracy in detecting uric acid and high probability to be used in the detection of uric acid in actual serum samples.2.The luminol-Al fluorescent nanomaterials are synthesized by the chelation effect between luminol and aluminum ions.The fluorescence intensity of the fluorescent nanomaterials are nearly 8 times stronger than that of luminol.The fluorescent nanomaterials can be used to detect the content of pyrophosphate and alkaline phosphatase.The principle of pyrophosphate and alkaline phosphatase is following:the coordination ability of aluminum ion and pyrophosphate(PPi)is stronger than that of aluminum ion and luminol,which can prevent aluminum ion from interacting with luminol.So the presence of pyrophosphate can reduce the fluorescence intensity of luminol-Al fluorescent nanomaterials.Based on this principle,a highly sensitive fluorescence quenching analysis method for the detection of pyrophosphate is established with the detection range of 0.1-60?M and the detection limit of 21.4 n M.When PPi is hydrolyzed to phosphate(Pi)by alkaline phosphatase(ALP),the fluorescence intensity of luminol-Al fluorescent nanomaterials is enhanced due to the weaker coordination ability of aluminum ions with Pi than that of PPi.Therefore a highly sensitive fluorescence-enhanced analysis method for the detection of alkaline phosphatase has been established,with a detection range of 0.5-20 U/L.The recovery methods of standard additions are used to investigate the accuracy of the detection method and the feasibility of detecting alkaline phosphatase in actual serum samples.The recoveries range from97.20%to 101.20%.It indicates that the way of detecting alkaline phosphatase is high accuracy and is expected to be used in the detection of alkaline phosphatase in actual serum samples.Compared with other detection methods,the fluorescence method for detecting PPi and ALP has the advantages of high sensitivity,high selectivity,simple operation,etc.In addition,it has potential application value in the fields of biological analysis and early diagnosis of diseases.3.Using glutathione to reduce manganese dioxide nanosheets to divalent manganese ions to prevent the fluorescence resonance energy transfer between luminol-Al fluorescent nanomaterials and manganese dioxide nanosheets.So the fluorescence intensity of the nanomaterials quenched by the manganese dioxide nanosheets is restored after adding glutathione.Based on this principle,a highly sensitive fluorescence-enhanced analysis method for the detection of glutathione is established.The detection range of this method is 0.2-20?M and the detection limit is 45 n M.Compared with other methods,this method has high sensitivity.In addition,other ions and biomolecules(L-lysine,L-threonine,L-valine)compared with glutathione have little effect on the fluorescence intensity of luminol-Al fluorescent nanomaterials.It indicates that this method is used to detect GSH with high selectivity.The recovery methods of standard additions are used to investigate the accuracy of the detection method and the feasibility of detecting GSH in actual serum samples.The recoveries range from 97.90%to 101.69%,which indicate that this method of detecting GSH has high accuracy and is expected to be used in the detection of glutathione in actual samples.