Design And Construction Of Nanochannel And Its Application In Detecting Reactive Oxygen Species

Solid-state biomimetic nanochannels with good stability,easy to fabrication and modification,which make them perform significant prospects in practical applications.Reactive oxygen species(ROS)play vital roles in the normal activities of living organisms,nevertheless the abnormal concentration of ROS can cause damage to the body and induce various diseases.Therefore,it is of great significance for accurate detection of ROS in the diagnosis and prevention of diseases.Owing to the ROS containing many intermediate species and short life,it is a highly ambitious task to detect ROS with high selectivity.Therefore,it is of great significance to design a rapid,sensitive and highly selective nanochannel system for the detection of ROS.Herein,based on the characteristics of reactive oxygen species,hydrogen peroxide and peroxy nitrite ion responsive nanochannels were designed and fabricated by combining with organic molecular probes and nanomaterials.The main contents are as follow:1.Hydrogen peroxide(H₂O₂)is an important reactive oxygen substance.Abnormal concentrations of H₂O₂can cause DNA and protein damage.Therefore,it is of profound significance to design a highly selective and sensitive analytical method for the detection of H₂O₂.Herein,we desigin and construct a hydrogen peroxide responsive nanochannl by introducing of 4-(phenoxy methyl)phenylborate(PBAE)into nanochannels based on self-assembly method.The PBAE functionalized nanochannels performed high sensitive and selective detection of H₂O₂.By virtue of the specific recognition response of PBAE and H₂O₂,the surface charge density of nanochannels will accordingly change and affect the properties of ion transport,as such to achieve a highly sensitive and specific detection of H₂O₂.In addition,the system can be applied to the detection of H₂O₂in complex samples(eg.extracts from living cells).This work provides a new approach for the development of highly sensitive and selective sensors for the detection of ROS substances.2.Peroxynitrite(ONOO^-)plays pivotal roles in organisms,but superfluous ONOO^-can result in oxidative stress in cells and organs,which may induce various severe diseases and disorders.At present,most of nanochannels are fabricated through modifying functional molecules into the nanochannel surfaces by covalent bonds,which lack modularity,dynamism and flexibility,thus consequently hampering their reuse potential in practical applications.In this context,we proposed a facile and rapid method for the detection of peroxynitrite anions(ONOO^-)by utilizing unmodified nanochannels integrated with N-(4-Aminobutyl)-N-ethyl isoluminol(ABEI)capped gold nanoparticles(ABEI@AuNPs).Due to the different aggregation patterns of ABEI@AuNPs and ONOO^-before and after the reaction,the ions transport in confined space will also change and affect the ionic current.Based on the concept,the nanochannel system can realize the rapid,highly sensitive and selective detection of ONOO^-.It can achieve multiple times test for the ONOO^-by repeatedly adding ABEI@AuNPs to the nanochannel system.Moreover,the practicality of the present method was validated by the analyses of serum samples which showed good recovery rate,indicating that the method has a good application prospect in complex samples.This work paves a new pathway to develop a versatile platform for the detection of different biological small molecules and reactive species.