Study On Synthesis,Characterization And Application Of Copper Phosphide Nanoparticles

In recent years,nanotechnology has developed rapidly,playing a huge role in energy,catalysis and biomedicine.Due to the unique physical and chemical properties of nanomaterials,nanotechnology has been gradually applied in the field of medicine,opening up a new path for the development of nanomaterials.Photothermal therapy and photodynamic therapy are considered to be the most promising new tumor treatment methods for clinical applications,but photothermal materials for phototherapy have yet to be developed.Among the well developed photothermal materials,copper-based nanomaterials with local surface plasmon resonance properties have great potential in near-infrared light therapy.Among them,copper phosphide nanomaterials have good local surface plasmon resonance absorption characteristics in the near-infrared region,but there are few reports on the application of copper phosphide nanomaterials in biomedicine.In this study,we first used a temperature-controlled synthesis system with simple conditions to obtain copper phosphide nanoparticles that can well absorb by plasma Surface Plasmon Resonance(LSPR)in the near-infrared region.This method avoids the risk of high temperature synthesis in the past.First,sodium hypophosphite was used as phosphorus source to react with cuprous oxide,by adjusting the reaction temperature,Cu₃P NPs with high photothermal conversion efficiency under 808nm NIR irradiation was obtained.Biocompatible pCu₃P NPs nanoparticles were obtained by polyethylene glycol(PEG)modification.Then a series of physical and chemical characterization were carried out to prove the successful production of copper phosphide and its excellent physical and chemical properties.The particle size of Cu₃P NPs was 176.0±20.4 nm by transmission electron microscopy.The surface potential of pCu₃P NPs was-15.3±0.65 m V after polyethylene glycol modification.The photothermal properties and photodynamic effects of pCu₃P NPs were investigated.The results show that pCu₃P NPs can produce excellent photothermal effects under near-infrared light irradiation,and the photothermal conversion efficiency can reach 52.78%.In addition,under the irradiation of near-infrared laser,pCu₃P NPs can produce reactive oxygen radicals(ROS),which are hydroxyl radicals.In order to explore the potential of pCu₃P NPs for biomedical applications,we first attempted to use pCu₃P NPs in combination with photothermal and photodynamic therapy for tumors.The phototherapy of pCu₃P NPs was studied in vitro.It was proved that pCu₃P NPs could be effectively absorbed by4T1 cells and had little effect on the viability of 4T1 cells.However,pCu₃P NPs had obvious killing effect on tumor cells under near-infrared laser irradiation;Secondly,through the detection of corresponding stress proteins and the analysis of flow cytometry,it was confirmed that the cell killing was induced by the photothermal and photodynamic properties of pCu₃P NPs.Next,an animal model was constructed to explore the anti-tumor effect in vivo.Studies have confirmed that pCu₃P NPs can accumulate in tumor tissues and can effectively inhibit the growth of tumor tissues and destroy the structure of tumor tissues under the irradiation of near-infrared laser.Pathological results after treatment showed that pCu₃P NPs showed little pathological changes in the main organs of mice,and showed high biosafety in the short term.In conclusion,pCu₃P NPs is an effective new nano particle that can be used in the combined photothermal and photodynamic therapy of tumor.This study opens up a new direction for biomedical application of copper phosphide,provides a new material platform for photothermal and photodynamic therapy,and provides a new idea for personalized combined therapy of cancer in the future.