The Study Of Mussel-inspired Antifogging And Antibacterial Protein Coating

Mist is a major challenge in many biomedical applications,and can interfere the functions of the optical lenses in medical equipments(such as endoscope,etc.).Current antifogging methods are normally inconvenient and may have potential safety hazards.Therefore,simple and biocompatible antifogging coatings are highly desirable.In addition,bacterial contamination is another major issue in biomedical applications.The bacteria adhesion and growth,as well as the formation of biofilms on the biological materials surfaces can cause serious consequences.Current antibacterial coatings commently suffer from insufficient protection effect and limited functions.Thus,developing biocompatible kill-resistant dual-functional antimicrobial coatings are of great significance.Inspired by the universal adhesion characteristics of mussels and hydrophilicity of zwitterionic peptides,a recombinant protein coating containing mussel foot silk protein,polylysine(K),and polyglutamic acid(E)was designed and prepared through synthetic biology strategy,its antifogging properties were investiged and a dual-functional coating loaded with silver nanoparticles was further prepared.First,the mussel-inspired chimeric protein(MP-KE)was immobilized on the glass by a one-step to obtain the coatings with different protein concentrations.The contact angle of 4 mg/m L protein coating was <5°,which was a superhydrophilic surface.The MP-KE protein coating has outstanding light transmission and antifogging performance,the results of UV-vis scanning,cold-hot and hot-cold experients demonstrated that the transparency of the protein coatings were similar with bare glass slides(86%-91%).The MP-KE protein coating can also resist bacterial adhesion,98.1% E.coli and 99% S.aureus.The hemolysis rates of protein coatings were <0.18%,the viabilities of co-cultured cells were >96%,and the blood coagulation index(BCI)were also 14% higher than the control,which indicated that MP-KE protein coatings have good blood compatibility and low cytotoxicity.Next,silver nanoparticles were introduced as a bactericide to develop kill-resistant coatings basing on the resistance bacterial adhesion property of the mussel-inspired chimeric protein.The MP-KE protein coating was loaded with silver nanoparticles under UV light.The inhibition ranges of E.coli and S.aureus caused by the protein coating containing silver nanoparticles were 19.97 and 17.60 mm,and the proliferation of high concentrations bacteria(OD600=0.1)were also effectively resisted for 24 h.And the loaded silver nanoparticles did not interfere the antibacterial properties,the amount of bacterial adsorbed in the coating was merely2.1% and the coating can effectively release the attached bacteria.In addition,the silver-loaded MP-KE protein coatings showed good biocompatibility,the hemolysis rates of them met the clinical application requirements(HR <5%),and the viabilities of the co-cultured cells were >92%.In summary,the mussel-inspired chimeric protein coating shows excellent antifogging and antimicrobial performance,and the coating loaded by silver nanoparticles possesses kill-resistant function.The introduction of mussel foot silk protein enables easy preparation of the coatings,a variety of protein coatings can be developed by editing functional modules,and providing broad poteintials applications.