Facile Synthesis Of Au@Mn₃O₄ Magneto-plasmonic Nanoflowers For T₁-weighted Magnetic Resonance Imaging And Tumor Photothermal Therapy

Cancer is life threaten disease which spreads across the world major cause of deaths both in males and females.Traditional therapy approaches such as radiotherapy,chemotherapy,and surgery have many drawbacks and cause collateral damages to healthy cells/tissues.A phototherapy treatment mechanism,especially photothermal therapy(PTT)is the most capable therapy approach.Photothermal therapy holds great capabilities to vanish the restrictions and aftereffects of traditional therapy methods.Indeed,the major advantage of theranostics is the capability to incorporate multiple functionalities in an agent,which can be used for imaging and therapy.Magnetic nanoparticles/nanohybrids are of paramount significance because of their varied biomedical applications.In this thesis,one type of magnetic nanohybrid nanoparticles-based biomaterials is fabricated and investigated their theranostics applications in breast cancer(4T1 cell line).Integration of advanced diagnostic contrast agents with versatile therapeutic drugs is an effective method for cancer treatment.However,combining various biocompatible theranostics modalities into single platform at nanoscale is a challenging assignment.In this thesis,a simple chemical synthetic route for producing homogeneous hybrid nanoflower shaped morphology based on Au@Mn₃O₄ magneto-plasmonic nanomaterials has been reported.The synthetic mechanisim of the nanoflowers is well matched with the heteroepitaxial growth phenomena by which the nano-petals of Mn₃O₄ generated on the surface of Au core.Food and drug administration(FDA)in USA approved triblock polymer Pluronic F-127 was used to enhance the biocompatibility of Au@Mn₃O₄ hybrid nanoflowers.The hybrid junction revealed promising optical and magnetic properties and the prepared Au@Mn₃O₄ nanoflowers exhibited strong NIR absorption to produce excellent photothermal efficacy under irradiation with 808nm NIR laser(1.2 W/cm²)as well showed the significant T₁-weighted MR image enhancement in vitro and in vivo.The histopathology assessments indicate that the toxicity of nanoflowers to major organs is negligible.As a result,hybrid Au@Mn₃O₄ nanoflowers exhibited great potential in T₁-weighted MR-imaging and photothermal therapy,which opened up new possibilities of synthesizing novel bio-compatible,homogeneous and shape controllable nanostructures for multifunctionally biomedical applications.Finally,this work highlights the huge potential of magnetically hybrid nanoparticles in theranostics applications for T₁-weighted imaging-guided photothermal cancer therapy.