Quorum Sensing Regulates The Oxidative Metabolism Of Saccharomyces Cerevisiae

Saccharomyces cerevisiae suffers oxidative damage during the process of growth and metabolism,and the quorum sensing mechanism is also accompanied by the process of microbial growth and metabolism.Therefore,it is necessary to explore the role of quorum sensing in the oxidative metabolism of S.cerevisiae.The research content of this subject mainly includes the regulation of quorum sensing on the reative oxygen content and oxidative tolerance of S.cerevisiae,the analysis of quorum sensing on the oxidative damage of S.cerevisiae,the transcriptomic analysis of quorum sensing on the regulation of the whole genome of S.cerevisiae,Regulation of RCK1 gene in mitogen-activated protein kinase(MAPK)pathway on oxidative metabolism of S.cerevisiae.The process is as follows:First of all,explore and study the regulation of quorum sensing on reative oxygen content and oxidation tolerance of S.cerevisiae.From the growth curve of wild bacteria and mutant bacteria,it can be concluded that the lack of quorum sensing causes the mutant bacteria to grow more slowly than the wild bacteria;The experimental results of culturing wild bacteria and mutant bacteria with dialysis bags show that there is a certain relationship between quorum sensing and the reative oxygen content in cells.In addition,the oxidation tolerance test of wild bacteria and mutant bacteria and the oxidative tolerance test of exogenous quorum sensing to wild bacteria and mutant bacteria showed that:The lack of quorum sensing leads to reduced tolerance of S.cerevisiae cells.In short,the experimental results show that quorum sensing is related to the oxidative metabolism of S.cerevisiae.Secondly,explore the analysis of quorum sensing on the oxidative damage of S.cerevisiae.It mainly studies the relative conductivity,cell swelling rate,mitochondrial membrane potential,and lipid oxidation level of wild and mutant S.cerevisiae.The experimental results show that the lack of quorum sensing causes the cell membrane of S.cerevisiae to receive a certain degree of oxidation.damage.Then,from the transcriptomics analysis of quorum sensing on the whole genome regulation of S.cerevisiae,it was found that there were 134 differential genes between wild strains and mutant strains,of which 79 were up-regulated genes and 55 were down-regulated.At the same time,fluorescence quantitative RCR technology was used to verify the accuracy of the transcriptome data.Finally,explore the regulation of the RCK1 gene on the oxidative metabolism of S.cerevisiae in the MAPK pathway.RCK1 gene is one of the protein kinases activated by MAPK in S.cerevisiae,which can play a role in anti-oxidation and resistance to metal stress.By knocking out the differential gene RCK1,the results indicate that quorum sensing molecules may affect the antioxidant effect of S.cerevisiae by affecting the MAPK pathway.All in all,the research of this subject can help us further study and discover the influence of quorum sensing on the growth and metabolism of S.cerevisiae,so that quorum sensing can be used rationally.