Study On Functionalized Modified LMSNs Nanoparticles As CoQ10 Delivery Carrier

Coenzyme Q10(CoQ10)plays an important role in mitochondria,participates in the electron transport chain of cellular respiration,activates cell metabolism,reduces the oxidative damage to cell membrane caused by peroxidation and free radicals in the body,and is more effective than vitamin B and E in inhibiting skin lipid peroxidation and improving anti-aging effect.It is also used as an adjuvant in the treatment of diabetes,gastric ulcer and cancer,and is an important functional food and dietary supplement.However,the existing conventional dosage forms of CoQ10,such as tablets,capsules and injections,have low bioavailability in vivo,so there is a broad prospect of using nanotechnology to improve the storage stability and bioavailability of CoQ10.Both Liposome and MSNs can improve the stability of CoQ10,so the Liposomes-coated MSNs composite nanoparticles were prepared by thin film dispersion method as the delivery carrier of CoQ10.CS,TPGS and Doca were used as functional modifiers to modify the nanoparticles to increase their retention time in the intestinal tract and the uptake efficiency of intestinal cells,so as improve the bioavailability of CoQ10.The appearance and morphology of the composite nanoparticles were evaluated by transmission electron microscope and Malvern particle size analyzer.The prepared composite nanoparticles had small particle size and good dispersion,and Liposomes could be successfully coated on the surface of MSNs.The physical and chemical properties of nanoparticles were further evaluated by FT-IR,DSC,Raman spectroscopy and X-ray diffraction.It was confirmed that Liposomes could be successfully coated on the surface of MSNs,and TPGS,CS and Doca could be successfully modified on the surface of nanoparticles as functional modifiers.The modified nanoparticles have stable physical and chemical properties and can be used as excellent delivery carriers for CoQ10.A rapid and efficient method for the determination of CoQ10 was established,and the linear range and accuracy of the CoQ10 detection method were studied.It was proved that the established CoQ10 detection method was fast,accurate and efficient.Using Caco-2 cells as intestinal model,the cellular transport and uptake kinetics of LMSNs were analyzed by flow cytometry to investigate the relationship between the effects of various factors on the pathway of LMSNs in and out of cells and the promotion of CoQ10 accumulation and absorption,in order to clarify the deep mechanism of nutrient-encapsulated composite nanoparticles being transported and absorbed at the cellular level.Adult SD rats were used as model animals to evaluate the intestinal uptake efficiency of different modified LMSNs.The results showed that the constructed composite nanoparticles could significantly increase the CoQ10 uptake of Caco-2 cells,and the modified nanoparticles showed extremely high cell uptake,which could overcome many obstacles of intestinal epithelium during oral administration of CoQ10.The composite nanoparticles as an oral delivery system of functional food has a very broad development prospect,and can provide new ideas and choices for the design and development of functional food nanoparticles.