| The preservation of fresh cut flowers is a promising industry with great potential and great economic value.It has been known that fresh cut flowers gradually change color,wilt and wither after harvest,which affect the quality and storage of fresh cut flowers,and then affect the ornamental quality.In the past three decades,it had been found that nitric oxide（NO）,as a signal molecule that plays important roles in seed germination,flower formation,fruit ripening and senescence,is also involved in delaying the senescence of fresh cut flowers.Hydrogen gas（H2）is a newly discovered gas signal molecule,which could participate in the regulation of plant growth,development and senescence,and could improve the tolerance of plants to stress.Usually,hydrogen is mainly used in plants in the form of hydrogen-rich water（HRW）.Magnesium hydride（MgH2）is a kind of magnesium-based hydrogen storage material with potential practical value in medicine and hydrogen energy battery,and its hydrogen storage density could be as high as 7.6wt%.Usually,MgH2 could react with water and release hydrogen slowly,and the dissolved hydrogen content of 0.01g/L MgH2 in water can reach 390.4 ppb in 0.5 h.Compared with the traditional method of producing hydrogen-rich water,the preparation of hydrogen-rich water by MgH2 is more time-saving and convenient.So far,there had been no report on the application of MgH2 in agricultural production.In view of the above problems,this paper first explored the role of MgH2 in the preservation of Chinese rose,carnation and lisianthus through physiological experiments,and found that MgH2 could prolong the vase life of the three fresh cut flowers and improved its ornamental quality.At the same time,from the point of view of NO signal molecules,this paper explored the regulatory mechanism of NO participation in MgH2 on the preservation of fresh cut flowers.The main findings are as follows:（1）Three kinds of typical cut flowers（Chinese rose,carnation and lisianthus）were selected and treated with MgH2（0.0001 g/L,0.001 g/L and 0.01 g/L）.It was found that MgH2 had fresh-keeping effects on three kinds of fresh cut flowers,which were mainly reflected in prolonging vase life,increasing flower diameter,increasing relative water content and antioxidant capacity.Among them,the best utility concentration of MgH2 was 0.001 g/L.（2）By selecting fresh cut Chinese rose,using NO scavenger（PTIO）and nitrate reductase（NR）inhibitor（Tungstate）,it was found that it could accelerate the senescence of fresh cut rose and lead to water loss,wilting and withering of petals of fresh cut Chinese rose,suggesting that endogenous NO plays an important role in the preservation of fresh cut flowers.Further study found that the regulatory effect of MgH2 on the preservation of cut Chinese rose could be blocked by PTIO and Tungstate,along with the decrease of endogenous NO.The above results suggested that NO may be involved in the regulation of MgH2 on the preservation of cut rose.（3）Based on the above results,a fresh cut flower preservative containing magnesium dihydride was applied,which can promote the growth and opening of the bud and effectively prolong the vase life of fresh cut flowers.The fresh cut flower preservative is applying for a national invention patent,the application number is:202010193539.9.In conclusion,using Chinese rose,carnation and lisianthus of fresh cut flowers as materials,it is found that MgH2 can be used to keep cut flowers fresh,and NO may be the downstream signal molecule of the above MgH2 effect.In addition,by applying for the national invention patent related to MgH2（Patent application number:202010193539.9）,it provides a theoretical basis for the practical application of magnesium dihydride. |
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