Synthesis Of Zinc-based Metal Framework Nanomaterials And Their Appplication On Wound Healing

Metal-organic frameworks(MOFs)have gained increasing attention in the field of biomedicine due to their unique properties such as tunable pore size,good biocompatibility,and excellent drug-loading capacity.MOFs are considered as the third generation of antibacterial agents due to the following advantages.(i)The central metal nodes of MOFs with antibacterial activity such as Ag,Cu,Ni and Zn show the characteristics of continuous release of metal ions;(ii)Organic ligands in MOFs(such as porphyrin derivatives,imidazole salts,etc.)show good antibacterial activity and biocompatibility;(iii)The high specific surface area and large porosity of MOFs are favorable for the effective packaging and transportation of antibacterial substances such as metal nanoparticles and biological macromolecules;(iv)Most MOFs show good stability and low biological toxicity in organisms.In this work,a new MOF(MTV-Zn-MOF)was synthesized by using natural antibacterial materials curcumin and 1,4-benzoic acid polyether polymer(L8)as ligands and Zn²⁺as metal connecting group.The synthesized MTV-Zn-MOF shows appropriate stability and can achieve the sustained release of curcumin and Zn²⁺from MTV-Zn-MOF(6.08?g m L^-1and 5.68?g m L^-1)within 24 h.Moreover,MTV-Zn-MOF exhibits good biocompatibility and low hemolysis rate at low concentration(125%)?g m L^-1),which can inhibit the growth of Staphylococcus aureus and Escherichia coli for ultra long time of 24 h.In vitro and in vivo experiments show that MTV-Zn-MOF can effectively inhibit the growth of Staphylococcus aureus and promote wound healing.This work can provide a powerful and novel antibacterial nanoplatform for antibacterial wound healing.A new multimodal antibacterial nanoplatform was further developed based on bioactive Zn-MOFs and Ti₃C₂T_xnanosheets for bacterial infection wound healing.Zn-MOF with accordion-like nanosheet structure extremely high specific surface area enabled the sustained release of curcumin(9.40?g m L^-1)and Zn²⁺(2.78?g m L^-1)within 24 h,while Ti₃C₂T_xnanoparticle enables the Zn-MOF@Ti₃C₂T_xcomposite with excellent photothermal conversion ability and excellent biocompatibility.The temperature of Zn-MOF@Ti₃C₂T_xsolution can raise to 49.7°C within 15 min under NIR 808 nm laser irradiation at a dose of100?g m L^-1with a photothermal conversion efficiency of 38.2%.Besides the PT effect,the Zn-MOF@Ti₃C₂T_xcan also generate reactive oxygen species such as¹O₂,which endow it with photodynamic therapy(PDT)effect.The research shows that Zn-MOF@Ti₃C₂T_xthe platform can realize rapid inactivation of bacteria through multi-mode antibacterial mechanisms,namely Zn²⁺and curcumin release,photothermal therapy(PTT)and photodynamic therapy(PDT).This work provides a multimodal antibacterial platform with excellent prospects for wound healing.