Preparation Of Graphdiyne Based Nanocomposites With Piezoelectric Response And Their Biological And Environmental Applications

Natural enzymes are difficult to produce on a large scale due to their high cost,difficult storage and easy inactivation.Nanozymes with low cost and high stability have gradually attracted the attention of researchers.Among a variety of nanozymes,peroxidase can catalyze H₂O₂ to generate ROS,which have important applications in many fields such as antibacterial,sensing,and catalysis,and thus have received extensive and indepth research.Carbon-based peroxidases have excellent chemical stability and easily regulated catalytic activity,which have great application prospects in the fields of catalysis,biology and environment.However,carbon-based peroxidases have relatively low intrinsic catalytic activity,limiting their practical application.Therefore,it is of great significance to improve the activity of carbon-based peroxidase.In this work,znic oxide nanorod@garphdiyne nanosheets(ZnO@GDY NRs)and sulfur doped graphdiyne(SGDY)with piezoelectric response were synthesized using graphdiyne(GDY).Their peroxidase-like activities were investigated,and they were used in antibacterial and wastewater treatment.The main contents are as follows:1.ZnO NRs were synthesized by a simple solvothermal method,and GDY were grown on their surfaces by in situ growth method to obtain ZnO@GDY NRs.The piezoelectric responses of ZnO@GDY NRs were characterized by piezoelectric force microscope(PFM).And then,taking the rhodamine B degradation experiment as a model,the enhanced peroxidase-like activity was characterized by degradation kinetic analysis under ultrasonic irradiation and the intrinsic mechanism was explored by density functional theory(DFT)calculation and COMSOL simulation.Based on their excellent catalytic activities,the antibacterial activity of ZnO@GDY NRs was tested in vitro,and the antibacterial efficiency reached 99.999%.Then,a mouse wound infection model was constructed and a flexible skin patch was designed using ZnO@GDY NR as an antibacterial agent to promote wound healing,which also showed a good biocompatibility.2.SGDY was synthesized by a simple hydrothermal method using GDY powder as raw material.SGDY has good piezoelectric response and peroxidase-like activitie.Using the oxidation experiment of TMB as a model,steady-state kinetic analysis of SGDY was carried out,confirming that SGDY has piezlelectric enhanced peroxidase-like activite.The underlying mechanism of SGDY was investigated by electron paramagnetic resonance analysis and DFT calculation,SGDY was applied to the degradation of dyes and antibiotics.Finally,a laboratory scale device is designed to treat a variety of sewage,and its TOC removal efficiency is higher than 40%.