Biomimetic Nanocarriers For Targeted Delivery And Cell Diagnosis And Treatment

So far,the application of biomimetic nanomaterials in the field of drug delivery and cell diagnosis and treatment has been widely concerned and applied,and more and more researchers have carried out in-depth exploration and development of biomimetic nanomaterials.Unlike traditional nanomaterials,as a result of biological molecules(including biological membranes,proteins,nucleic acids,etc.)on the modification of nanomaterials,biomimetic nano-materials are endowed with more advantages,such as better biocompatibility,more accurate targeting,extension of drug circulation,etc.,making it appear in the application of better security and treatment effect.With the development of research,various biomimetic nanomaterials have been widely used.How to construct more sensitive and efficient biomimetic nanomaterials has become the focus of researchers in the field of nanomedicine.Using cell membrane and protein as models,we constructed biomimetic nanocarriers for targeted delivery and cell diagnosis and treatment.The specific research contents are as follows:1.Construction of membrane-coated Au-Fe₃O₄ nanoagent(AFTP@CM):Taking the synthesized gold nanoparticles as the core,Au-Fe₃O₄ nanoparticles with micropores on the surface were obtained through a series of reactions,namely AF.AFTP was obtained by co-incubation of AF with TA and PMA.Finally,the prepared MCF-7 breast cancer cell membrane(CM)was coated on the surface of AFTP to obtain AFTP@CM.The targeting capabilities of cancer cell membranes enable AFTP@CM to pinpoint tumor sites.After entering cells,TA and PMA,as drugs promoting Fenton response,produced enhanced CDT effect and effectively improved cytotoxicity.As a composite of Au nanomaterials and Fe3O4nanomaterials,Au-Fe₃O₄ has strong PTT effect.From experimental data,AFTP@CM has performed well in targeted drug delivery and cell therapy.Compared with monotherapy,the combined treatment strategy of enhanced chemo/photo therapy showed better therapeutic effect,providing a new perspective and idea for the application of biomimetic nanomaterials in the field of nanomedicine.2.Construction of a nuclear targeted delivery system for si RNA using lysosomal escape protein nanocarriers:Ferritin deficiency(Apn)with aptamer AS1411 modified on the surface cleaved with p H reduction and mixed with si RNA/NLS and HA peptides.By increasing p H and regrouping ferritin,the drug was cleverly encapsulated and loaded to obtain Apn/siRNA/NLS/HA/Apt probes.Under the action of targeting,the probe quickly enters the cell and comes to the lysosome site.Apn/si RNA/NLS/HA/Apt with the nature of lysosome escape protects the structure of si RNA and enters the nucleus to play a role under the action of nuclear localization peptide.The Apn/si RNA/NLS/HA/Apt probes are easy to synthesize and have a rapid and effective action process,which provides a new idea for bionic nanosystem in the field of drug delivery and gene therapy.In conclusion,based on the bionic nanocarriers,this paper combines the advantages of cancer cell membrane,ferritin deficiency and other biomolecules and nanomaterials to conduct in-depth exploration of targeted drug delivery and cell diagnosis and treatment,providing a new perspective and theoretical basis for the research of bionic nanocarriers in the field of nanomedicine.