Preparation Of FePt And MnO₂ Nanocascade Reactor And Its Application In Tumor Treatment

In recent years,reactive oxygen species(ROS)-based cancer treatment strategies including photodynamic therapy(PDT),sonodynamic therapy(SDT),and chemodynamic therapy(CDT)have received extensive attention.Although these treatment strategies have achieved certain effects in cancer treatment,the inherent drawbacks of a single treatment strategy greatly reduce their therapeutic effect.In addition,many therapeutic strategies require high oxygen levels or specific external stimuli to generate adequate ROS.Therefore,it is urgent to develop multifunctional synergistic agents to resolve the deficiency of monotherapy and enhance the antitumor effect.In this paper,a novel multifunctional nanotherapeutic drug BSA@GOx@FePt@Mn O₂(BGMFP)was prepared by integrating glucose oxidase(GOx),bovine serum albumin(BSA),manganese dioxide(Mn O₂)and platinum iron nanoparticles(FePt NPs).For the synthesis of the nanomaterials,FePt NPs were firstly prepared by the thermal decomposition and thermal reduction procedure.After dispersed in oleic acid solution,the potassium permanganate solution was added and the flowerlike Mn O₂@FePt was obtained.Finally,the GOx and BSA were added to the Mn O₂@FePt mixture and BGMFP was obtained via coordination bonding(Mn-N and Mn-O)and electrostatic adsorption effect.In cancer microenvironment,the intratumoral H₂O₂ could be transformed to O₂ catalyzed by Mn O₂ to alleviate the anoxia condition and advance the catalytic ability of GOx to accelerate the consumption of glucose.Hence the cancer cells will be“starvation”due to the blocking of the energy supply.On the other hand,the H₂O₂ produced during the consumption of glucose would in turn increase the level of H₂O₂.Therefore,the above reactions could be mutual promotion of each other to enhance their own catalytic effect.Additionally,the FePt NPs could decompose to Fe²⁺to convert H₂O₂ to the toxic ROS,subsequently resulting in the cell apoptosis.Hence,the H₂O₂generated during the consumption of glucose could boost the fenton reaction efficiency by increased the level of H₂O₂.The structure and constitution of the materials were characterized through TEM and ICP-MS,et al.The results in cancer cells revealed that BGMFP have excellent biocompatibility and could kill cancer cells effectively.The anticancer results in mice further demonstrated that BGMFP possessed favorable tumor-targeting property and remarkable tumor inhibition capacity through enhancing the starvation therapy and CDT.Therefore,BGMFP could be a promising nanomedicine for synergetic cancer treatment.