Application Of Synchronous Fluorescence Analysis In Nucleic Acid Fluorescent Probes

Nucleic acid fluorescent probes are characterised by good selectivity,high sensitivity and a wide range of applications.However,there are some drawbacks when using fluorescence emission spectroscopy for the quantitative analysis of targets.Firstly,the scattering light interferes with the fluorescence spectrum in many cases,and secondly,the one-step simultaneous quantitative detection of multi-components can not be realized when the multi-components are detected.Based on this,this experiment designed 3 kinds of multi-color nucleic acid fluorescent probes to realize the ratio quantitative detection of single target and multi-targets by synchronous fluorescence analysis.A ratiometric fluorescent probe that specifically recognized Ag⁺was constructed using the fluorescent dye TAMRA-labeled Ag⁺nucleic acid aptamer and the nucleic acid dye SYBR Green I.A highly selective rapid fluorescence quantification method for Ag⁺was established using this fluorescent probe;A multicolor fluorescent probe was constructed using the bimetallic organic framework nanomaterial(BNMOFs)Cu/Ui O-66 and three fluorescent dye-labelled aptamers of acetamiprid,atrazine and carbendazim,using which a one-step simultaneous quantitative method for the detection of three pesticides was established;A multicolour nucleic acid fluorescent probe was constructed using BNMOFs nanosheets of Co/Fe(1:1)MOFs and complementary strands(c DNAs)of four different target deoxyribonucleic acids(DNAs)labelled with four fluorescent dyes,which was used to establish a one-step simultaneous quantitative detection method for four DNAs.Accurate trace quantification of silver ions in water using the nucleic acid dye SYBR Green I and fluorescent dye-labeled nucleic acid aptamer;rapid one-step quantification of three pesticides in cabbage samples using the bimetallic organic framework nanomaterial Cu/Ui O-66 and three fluorescent dye-labelled aptamers of acetamiprid,atrazine and carbendazim;rapid one-step quantification of four DNA fragments in complex matrices using Co/Fe(1:1)MOFs nanosheets and four c DNAs labelled with four fluorescent dyes.In this experiment,three multicoloured nucleic acid fluorescent probes were designed and three different quantitative assays were established using synchronous fluorescence analysis.The rapid quantitative detection of silver ions in water,the one-step quantitative detection of acetamiprid,atrazine and carbendazim in cabbage and four DNA fragments in complex matrices were achieved respectively.These methods have the advantages of simple operation,short detection time,high sensitivity and low detection limits.