Synthesis And Glucose-Responsiveness Of Synthetic-Lectin Polymer Microgels

Glucose is an intermediate product of biological metabolismand energy substance,and is closely related to diseases such as cancer and diabetes.Therefore,how to detect glucose rapidly,accurately and non-invadably in polar environment has been an important research topic and a huge challenge.Glucose responsive microgels are expected to be used to design and build systems that can sustainably detect glucose and output feedback.This paper is centered around glucose responsive microgels to launch the research work,focusing on the synthesis of microgel-related response primitives and gel skeletons.First of all,referring to the research work of mimic lectins reported in the literature,we focus on the difficulty of organic total synthesis of mimic lectins,and optimize the synthetic route and experimental scheme.At the same time,we try to construct a π surface with phenyl units,amide bond for hydrogen bonding interaction to explore the possibility of similar structure,in the hope that these mimic lectin structures can complement the "all-equatorial" substitution pattern of carbohydrate.Secondly,using the synthesis lectin(sLectin)with the different structures as responsive primitives,to be loaded into microgels skeleton in different ways to prepare new glucose responsive microgels.We also detect the glucose responsiveness of these microgels by particle size changes and optical characterization preliminarily.Our research lays the foundation for further research on glucose responsiveness and its application in glucose detection.(1)We synthesised a dendritic macrocyclic moleculeas the first through 4 ways.Then it was physically wrapped in Poly(N-isopropylacrylamide)(pNIPAM)microgels.In the aqueous solution,The dynamic light scattering test(DLS)revealed that the-microgels will swell by increasing the concentration of the glucose.And turbidity studies show that the extinction of the microgel dispersion decreases.The result also supported the interaction between-microgels with glucose.(2)Carboxylic compounds with double bonds and diamine anthracene were successfully synthesized.Firstly,carboxylic compounds were polymerized into microgels,Secondly,the anthracene-containing diamine compounds were added into the as-synthesized polymer micorgels to forme a cavity through ionic bonds.Two kinds of microgels were successfully applied to detect different concentrations of glucose in urine.The measured UV-vis absorption and fluorescence emission spectra both showed good regularity,which provides more possibilities for the structural design of sLectin.(3)According to the idea of the guest-host interaction,a new contained macrocycle was designed,which double bonds were introduced in advance in order to covalently polymerize macrocyclic compounds into microgels.With the increasing of glucose concentration in aqueous solution,the volume phase transition behavior of the polymer microgels showed greater swelling.This result indicated that microgel which covalently polymerized by small molecule receptors have a wider range of concentration and higher sensitivity to glucose.