Synthesis And Performance Of A Novel Fluorescent Probe Based On Triphenylamine

In the past 25 years,the underlying amino acid mechanism of action has only begun to emerge,and protein malnutrition will reduce the concentration of most amino acids in the plasma.Recent research results have shown that regulating the content of amino acids in the human body plays an important role.Due to the negative impact of the imbalance and antagonism between amino acids on nutrient intake and utilization,the content of intestinal or parenteral amino acids should be carefully formulated to maximize Gain benefits.Such measures should be based on the following knowledge:the biochemistry and physiology of amino acids,the role of amino acids in the immune response,the nutritional and pathological status of the individual,and the expected treatment outcome.These nutrients have broad prospects in improving health and preventing infectious diseases in animals and humans.In this paper,we have studied the response of triphenylamine derivatives to different amino acids in depth.Through the Schiff base reaction,molecular probes containing guanidine groups and hydroxyl groups were constructed,and the compounds TNB,TNN,and TCN were synthesized.Through Schiff base reaction,molecular probes containing guanidine groups and hydroxyl groups were constructed,and compounds TNB,TNN,TCN were synthesized.Characterized by ultraviolet（UV）,mass spectrometry（MS）,nuclear magnetic（NMR）and other test methods,all showed that the three compounds have been successfully synthesized.Studies have found that the compound TNB can only produce fluorescence when mixed with Trp.This may be because TNB has a flexible structure and can accommodate Trp molecules.In addition,TNB has more hydroxyl groups and benzene rings,which can form Proton transfer with Trp,thus making the molecules restricted internal movement produce fluorescence.The environment of different p H values??will also affect the fluorescence response of TNB to amino acids.Under strong acid or base conditions,the hydroxyl group of the compound will be destroyed.When tryptophan is added,the compound TNB does not produce fluorescence.In order to make the experiment more reliable,we designed and synthesized a compound TNN that does not contain a hydroxyl group.Through UV testing,we found that after a period of reaction between TNN and all amino acids,no new peaks appeared,and the NMR data hardly changed（amino acids are insoluble in DMSO）.Therefore,we believe that TNB produces fluorescence after adding tryptophan,and the hydroxyl and aromatic heterocycles play a decisive role.We use synthetic TNB raw materials can be used to detect H₂O₂and ClO^-,this compound is based on the derivative of triphenylamine tris（4-aminophenyl）amine（NBN）.This compound is based on the derivative of triphenylamine,tris（4-aminophenyl）amine（NBN）.The detection of H₂O₂and ClO^-is of great significance to the entire society.H₂O₂is known as an oxidative substance,which plays a very important role in the cells of organisms such as humans or animals.The main role is related to cell migration,Proliferation,differentiation and immune response.Many related reports have confirmed that excessive H₂O₂in the human body will cause great damage to nucleic acids,proteins and biofilms,and it may also cause many intractable diseases.Therefore,it is very important to develop an effective probe for the detection of hydrogen peroxide concentration.On the other hand,the balance of hypochlorite ions in the human body is broken,which can also cause various diseases,such as cardiovascular disease and cancer.Therefore,it is particularly important to develop a rapid and effective method for detecting hypochlorite.All in all,this thesis designs and synthesizes the compound TNB,conducts in-depth comparison and research on its performance,and explores and explains their fluorescence generation mechanism.It provides theoretical support for the convenient and specific detection of amino acids,and has great practical significance in biology,health,and medicine.Moreover,the use of the compound NBN to detect H₂O₂and ClO^-is also very far-reaching for the entire society’s significance.