Response Of Datura Stramonium L. Experienced With Different Pollution To Toxic Metals And Salt Stress

Plants adapting the polluted environment,mean not only being able to actively respond to the polluted environment,but also adapting to the changes in site conditions and the new stress environment caused by pollution.Studying the ecological response of plants that have experienced polluted environment to related pollution and stress environment is an important starting point for in-depth understanding of the adaptability of plant pollution.There are few systematic studies in related research at home and abroad.Thus,this study takes Datura,a pioneer plant in the abandoned mining area of Yunnan,as the research object,and used Datura experienced with different pollution as experimental materials to analyze the response to the conditions of heavy metal lead,cadmium and salt stress in plants.Analyze the response of growth,reproduction and physiological that adapt to the polluted environment and the stress environment.The key research findings are following.（1）The ecological responses of Datura experienced with different heavy metal pollution showed: heavy metal pollution will reduce the plant height of Datura.The plant height of the M0 population（experienced without pollution）was higher than that of the M1 and M2 populations（two populations experienced with different pollution）.Under high-concentration heavy metal stress（Pb>1300 mg/kg and Cd>50 mg/kg）,the root length of Datura experienced without pollution was greater than that of populations experienced with different pollution,but the biomass is opposite（M1>M0）.From the perspective of reproductive characteristics,the pollen viability coefficients of Datura（M1 and M2）were the highest,as 31.84% and 44.47% respectively,which are smaller than that experienced without pollution.From the perspective of physiological characteristics,under lead stress and high-concentration cadmium stress（Cd 50 mg/kg）,the catalase activity and glutathione content of populations experienced with different pollution were significantly higher than those of Datura experienced without pollution.Under high concentration of heavy metal stress（Pb 1200 mg/kg,Cd 30 mg/kg）,the proline content of M1 population was significantly higher than that of M0 population;under Pb 1200 mg/kg,and Cd 30 mg/kg,the malondialdehyde content of M2 population was significantly higher than the M0 population.（2）The ecological response of Datura experienced with different pollution to salt stress showed : the plant height,stem diameter,and biomass of the Datura experienced with different pollution were higher than those without pollution,but the root length was the opposite.From the perspective of reproduction,salt stress delayed the flowering period of Datura,however,the effect on pollen vitality was not significant.From the perspective of physiological characteristics,the catalase activity and glutathione content of the M2 population were significantly higher than that of the M0 population at 60 days of salt stress;the M0 population had significantly higher malondialdehyde content at 120 days of salt stress in the M2 population.（3）The synchronicity of the response of Datura to heavy metal pollution and salt stress showed: under heavy metal stress,M0 and M2 showed more similar in morphology characteristics,while the characteristics of M0,M1,and M2 were not significantly different under salt stress;regression analysis found that after 60 days of lead stress,the first principal component of the morphological characteristics of the M2 population was significantly negatively correlated with the salt concentration（p=0.029）;At 60 days of salt stress,the first principal component of M1 population was significantly negatively correlated with salt concentration（p=0.045）;at 120 days,the first principal component of M0 population was significantly negatively correlated with salt concentration（p=0.045）.From the reproductive characteristics,under salt stress,the first principal component of reproductive characteristics of Datura M1 and M2 populations was significantly positively correlated with salt concentration（p=0.047 and0.043）;under lead stress,the first principal component of M1 population had a very significant positive correlation with lead concentration（p=0.005）.From the perspective of physiological characteristics,the physiological clustering results of the Datura populations showed that the physiological characteristics of the M1 and M2 populations were similar under lead stress.The regression analysis results showed that only when the cadmium stress was at 120 days,the physiological first principal component of M2 population had a very significant positive correlation with the cadmium concentration（p=0.015）.The discussion concluded that the growth parameters of the Datura population with pollution under heavy metal and salt stress are generally better than those of the Datura population without pollution,indicating that the plant has a synergistic adaptation phenomenon in adapting to heavy metal pollution and responding to salt stress.