Synthesis Of Luminescent Functionalized Conductive Polymer/carbon Nanocomposites And Its Analytical Application

Based on the excellent electrochemical performance,biocompatibility,stability and high conductivity of conductive polymer/carbon nanocomposites,which have been widely used in photocatalysis,supercapacitors and biosensors.In this thesis,three luminescent functionalized nanocomposites poly（anline-luminol）/GO,poly（pyrrole-luminol）/MWCNTs and poly（anline-luminol）/Au NPs/GO with excellent ECL properties were successfully synthesized by chemical oxidation.And then using poly（anline-luminol）/GO and poly（pyrrole-luminol）/MWCNTs nanocomposites as the nanointerface,two electrochemiluminescence（ECL）immunosensors for the detection of alpha-fetoprotein（AFP）and carcinoembryonic antigen（CEA）were constructed,respectively.At the same time,the rapid detection of AFP and CEA in human serum samples was realized.The main contents are as following:1.Poly（anline-luminol）/GO nanocomposite were prepared with a rapid and simple chemical oxidation method,and the nanocomposite was characterized,the results showed that poly（anline-luminol）/GO nanocomposite was successfully synthesized.Based on the rich amino groups,good film-forming and excellent ECL properties of the nanocomposite,by the glutaraldehyde crosslinking method anti-AFP was immobilized on the surface of poly（anline-luminol）/GO modified electrode,then a novel ECL immunosensor for detecting AFP was successfully constructed based on anti-AFP/poly（anline-luminol）/GO.The linear range of the ECL immunosensor for detecting AFP was 1.7×10^-12-1.7×10^-8mg/m L,and the low detection limit was 5×10^-13mg/m L.It was proved that the ECL immunosensor had a high sensitivity and a wide linear range.Finally,the immunosensor has been successfully applied to the quantitative detection of AFP in human serum samples.2.A novel ECL immunosensor for CEA detection was developed that using poly（pyrrole-luminol）/MWCNTs nanocomposite as the interface.Firstly,poly（pyrrole-luminol）/MWCNTs nanocomposite was prepared by chemical oxidation method,and the nanocomposite was characterized,the results showed that pyrrole and luminol were successfully loaded on the surface of MWCNTs.Then,the synthesized material was loaded on the electrode surface,and the anti-CEA was immobilized on the electrode surface with EDC/NHS as the crosslinking agent,the obtained sensor platform had strong and stable ECL signal.When CEA was captured by its antibody through the immunoreaction between CEA and anti-CEA,the intensity of ECL was decreased,thus the detection of CEA can be realized.The linear range of ECL immunosensor for the detection of CEA was 1.0×10^-13-1.0×10^-8mg/m L,and the low detection limit was 1.0×10^-14mg/m L.At the same time,the developed immunosensor has been successfully applied to quantify CEA in human serum samples.3.Poly（anline-luminol）/Au NPs/GO nanocomposite were successfully prepared by chemical oxidation and non-covalent bonding between aniline,luminol,Au NPs and GO.The experimental results showed that Au NPs and GO played a good catalytic role in the nanocomposite that makes the poly（anline-luminol）/Au NPs/GO nanocomposite have excellent electrochemical,chemiluminescence and ECL properties.At the same time,poly（anline-luminol）/Au NPs/GO nanocomposite contained abundant amino groups and the Au NPs in the nanocomposite can be combined with a variety of biomacromolecule,which can provide a good platform for the construction of sensors.Therefore,poly（anline-luminol）/Au NPs/GO nanocomposite can be used as nano-interfaces to develop a variety of ECL biosensors for detecting DNA,enzymes,proteins and other biomacromolecule.