The Construction Of Photoelectric Sensors And Its Application In The Detection Of Thrombin

Objective Based on the excellent conductivity of carbon materials and cadmium sulfide,to prepare nitrogen-doped biochar and composite materials with good photoelectric properties.It is prepared into a photoelectrochemical biosensor for high-sensitivity,simple and rapid analysis and detection of certain characteristics or markers of the environment and diseases.At the same time,by preparing photoelectric materials with superior photoelectric conversion performance,the electrodes are given good photoelectric conversion capabilities.Methods 1.Using the excellent conductivity of nitrogen-doped biochar composite materials to prepare porous biochar with larger specific surface area,And modified it on the glassy carbon electrode to make a sensitive,low detection limit biosensor,Used for simultaneous determination of hydroquinone(HQ)and catechol(CA).2.Prepare CdS materials with strong photoelectric signal,short reaction period and stable photoelectric signal through relatively simple steps.Use the form of assembled electrodes combined with a label-free detection strategy,the simultaneous rapid detection of thrombin in each sensing area is realized.3.Based on the special chemical activity of C60,synthesize derivatives with good water solubility and excellent photoelectric properties.And modified it on the ITO surface to prepare a photoelectric signal sensitive,bioelectrode.Link fibrinogen through label-free and self-assembly methods to achieve high-throughput detection of thrombin.Results Utilizing the excellent electrical conductivity of ITO,its surface modification has good photoelectric properties,and the biocompatible semiconductor material is divided into different partitions with perforated glue.A biosensor platform is constructed to quickly detect multiple targets,and has the advantages of high integration,wide linear range,low detection line,and stable anti-interference.Under optimized experimental conditions,different concentrations of thrombin have different cleavage abilities to the recognition sites on the biosensor.Part of the peptide chain is detached from the surface of the electrode,which affects the resistance of the electrode and changes the photocurrent or photoelectric potential,which can be used for the detection of thrombin activity.Here,the light excitation strategy can be used to detect the activity of different concentrations of thrombin in sequence.In addition,the photocurrent of the biosensor is stable and changes with the concentration of thrombin,and gradually stabilizes after a certain period of time.Conclusion Through the synthesis of water-soluble C60 photoelectric materials and the optimization of high-performance photoelectric materials fixed on the electrode surface to form a biosensor,Through different electrochemical detection methods such as CV,DPV,and i-t,it has been determined that the idea of using fibrinogen as a substrate peptide-specific site recognition and detection of thrombin is feasible,Preliminarily determine the qualitative and quantitative research and analysis method of the label-free photoelectrochemical biosensor for thrombin.The unique structural characteristics of thrombin are used to realize the high efficiency,high specificity and high stability of the biosensor with fibrinogen.Optimize the preparation conditions of high-performance fullerene water-soluble photoelectric active materials,and use simple and easy-to-obtain methods to prepare C60 photoelectric composite materials that are excited by visible light,have good water solubility,and are non-toxic and harmless.It is directly immobilized on the surface of the photoelectric material electrode through the hydroxyl affinity reaction of the ITO surface to realize an integrated,multi-target,and anti-interference photoelectric conversion biosensing platform and a screening method for the activity of biological reagents.Establish a new idea of label-free,multi-target analysis of thrombin concentration and enzyme activity detection.