Preparation And Application Of Nanocarriers Based On Spindle-shaped Hydroxyl Ferric Oxide And Its Antitumor Effect

For nearly a century,cancer has become the second leading cause of death in humans,and overcoming obstacles such as a short circular half-life and nonspecific systemic distribution is an ongoing challenge in cancer treatment.The development of cancer therapeutic platforms for nanoparticles(NPs)has also been extensively explored by different scientists.Photothermal/photodynamic therapy(PTT/PDT)and synergistic therapy strategies have been favored because of their low side effects and minimal invasiveness compared with chemotherapy and surgery.The effective delivery of highly effective photosensitizer and photothermal agent is the key of photodynamic and photothermal therapy.In the course of treatment,how to enrich the material to the tumor site and reduce the biological toxicity of the material is still an urgent problem to be solved.This paper aims to construct nanocarriers based on spindle-shaped hydroxyl ferric oxide and design nanocarriers with low biological toxicity.Iron hydroxyl oxide(FeOOH),an important component of iron oxides,has stable chemical properties,high specific surface area and fine particle structure,and is a class of nanostructured materials with great development prospects.Spindle hydroxyl iron oxide(FeOOH NSs)with controllable nanometer size,can pass the EPR(enhanced permeability and retention)effect in tumor position,so we modified hydroxyl by bovine serum albumin(BSA)iron oxide nanoparticles,to improve its biocompatibility,again by sunlight or light load reagent,so as to achieve the purpose of thermal treatment and light moving.In the second chapter of this paper,we constructed the FeOOH@BSA-Ce6 nanocomposite with core-shell structure,and the obtained nanoparticles have appropriate nanometer size and can be uniformly dispersed in the aqueous phase.The cytocompatibility CCK-8 assay shows that FeOOH@BSA-Ce6 is less cytotoxic in the presence of no laser irradiation,and can be endocytotic,and has a strong cell lethality under the irradiation of 660 nm laser.After the injection of FeOOH@BSA-Ce6 composite nanoparticles through the tail vein,ICP-MS test showed that the nanoparticles had a long blood circulation time and could be passively targeted to the tumor site through EPR effect.After laser irradiation,the tumor growth could be inhibited to a certain extent.Relevant in vitro and in vivo test results show that the composite nanoparticles have certain anti-tumor effect.In the third chapter,bovine serum protein(BSA)was used as a stabilizer for pyrrole polymerization and spindle-like hydroxyl ferric oxide(FeOOH NSs)was used as a pyrrole nanotemplate to construct spindle-like FeOOH@PPy composite nanoparticles.The average particle size of the obtained nanoparticles is about 100 nm,and it has good stability in the water condition.It has an obvious absorption peak at 808 nm.The results of photothermal performance test show that FeOOH@PPy composite nanoparticles can absorb 808 nm near-infrared light and convert it into enough heat to cause tumor cell apoptosis.In a 14-day4T1 breast cancer mouse model,FeOOH@PPy composite nanoparticles showed excellent antitumor effect.The analysis methods of tissue fraction showed that the composite nanoparticles had no obvious effect on normal tissues of mice,indicating that the composite nanoparticles could be used as photothermal therapy.